Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease

Kathryn L. Pothoven, James E. Norton, Kathryn E. Hulse, Lydia A. Suh, Roderick G. Carter, Erin Rocci, Kathleen E. Harris, Stephanie Shintani-Smith, David B. Conley, Rakesh K. Chandra, Mark Chang Hwa Liu, Atsushi Kato, Nirmala Gonsalves, Leslie C. Grammer, Anju T. Peters, Robert C. Kern, Paul J. Bryce, Bruce K. Tan, Robert P. Schleimer

Research output: Contribution to journalArticle

Abstract

Background Epithelial barrier dysfunction is thought to play a role in many mucosal diseases, including asthma, chronic rhinosinusitis (CRS), and eosinophilic esophagitis. Objective The objective of this study was to investigate the role of oncostatin M (OSM) in epithelial barrier dysfunction in human mucosal disease. Methods OSM expression was measured in tissue extracts, nasal secretions, and bronchoalveolar lavage fluid. The effects of OSM stimulation on barrier function of normal human bronchial epithelial cells and nasal epithelial cells cultured at the air-liquid interface were assessed by using transepithelial electrical resistance and fluorescein isothiocyanate-dextran flux. Dual-color immunofluorescence was used to evaluate the integrity of tight junction structures in cultured epithelial cells. Results Analysis of samples from patients with CRS showed that OSM mRNA and protein levels were highly increased in nasal polyps compared with those seen in control uncinate tissue (P 2-macroglobulin, a marker of epithelial leak, in localized nasal secretions (r = 0.4855, P

Original languageEnglish (US)
Pages (from-to)737-746.e4
JournalThe Journal of Allergy and Clinical Immunology
Volume136
Issue number3
DOIs
StatePublished - Sep 1 2015

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Oncostatin M
Epithelial Cells
Nose
Nasal Lavage Fluid
Eosinophilic Esophagitis
Nasal Polyps
Macroglobulins
Tissue Extracts
Tight Junctions
Bronchoalveolar Lavage Fluid
Electric Impedance
Fluorescent Antibody Technique
Cultured Cells
Asthma
Color
Air
Messenger RNA
Proteins

Keywords

  • atopic asthma
  • chronic rhinosinusitis
  • eosinophilic esophagitis
  • epithelial barrier
  • Oncostatin M
  • tight junctions
  • transepithelial electrical resistance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. / Pothoven, Kathryn L.; Norton, James E.; Hulse, Kathryn E.; Suh, Lydia A.; Carter, Roderick G.; Rocci, Erin; Harris, Kathleen E.; Shintani-Smith, Stephanie; Conley, David B.; Chandra, Rakesh K.; Liu, Mark Chang Hwa; Kato, Atsushi; Gonsalves, Nirmala; Grammer, Leslie C.; Peters, Anju T.; Kern, Robert C.; Bryce, Paul J.; Tan, Bruce K.; Schleimer, Robert P.

In: The Journal of Allergy and Clinical Immunology, Vol. 136, No. 3, 01.09.2015, p. 737-746.e4.

Research output: Contribution to journalArticle

Pothoven, KL, Norton, JE, Hulse, KE, Suh, LA, Carter, RG, Rocci, E, Harris, KE, Shintani-Smith, S, Conley, DB, Chandra, RK, Liu, MCH, Kato, A, Gonsalves, N, Grammer, LC, Peters, AT, Kern, RC, Bryce, PJ, Tan, BK & Schleimer, RP 2015, 'Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease', The Journal of Allergy and Clinical Immunology, vol. 136, no. 3, pp. 737-746.e4. https://doi.org/10.1016/j.jaci.2015.01.043
Pothoven, Kathryn L. ; Norton, James E. ; Hulse, Kathryn E. ; Suh, Lydia A. ; Carter, Roderick G. ; Rocci, Erin ; Harris, Kathleen E. ; Shintani-Smith, Stephanie ; Conley, David B. ; Chandra, Rakesh K. ; Liu, Mark Chang Hwa ; Kato, Atsushi ; Gonsalves, Nirmala ; Grammer, Leslie C. ; Peters, Anju T. ; Kern, Robert C. ; Bryce, Paul J. ; Tan, Bruce K. ; Schleimer, Robert P. / Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. In: The Journal of Allergy and Clinical Immunology. 2015 ; Vol. 136, No. 3. pp. 737-746.e4.
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AU - Harris, Kathleen E.

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