Oncogenic forms of p53 inhibit p53-regulated gene expression

Scott E. Kern, Jennifer A. Pietenpol, Sam Thiagalingam, Albert Seymour, Kenneth W. Kinzler, Bert Vogelstein

Research output: Contribution to journalArticlepeer-review

Abstract

Mutant forms of the gene encoding the tumor suppressor p53 are found in numerous human malignancies, but the physiologic function of p53 and the effects of mutations on this function are unknown. The p53 protein binds DNA in a sequence-specific manner and thus may regulate gene transcription. Cotransfection experiments showed that wild-type p53 activated the expression of genes adjacent to a p53 DNA binding site. The level of activation correlated with DNA binding in vitro. Oncogenic forms of p53 lost this activity. Moreover, all mutants inhibited the activity of coexpressed wild-type p53, providing a basis for the selection of such mutants during tumorigenesis.

Original languageEnglish (US)
Pages (from-to)827-830
Number of pages4
JournalScience
Volume256
Issue number5058
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • General

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