Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells

Jung Sik Kim, Heather Crooks, Tatiana Dracheva, Tagvor G. Nishanian, Baljit Singh, Jin Jen, Todd Waldman

Research output: Contribution to journalArticle

Abstract

Somatic cell gene targeting was used to create an isogenic set of human colon cancer cells that differs only in the presence or absence of their endogenous activated β-catenin oncogene. Affymetrix Genechip expression profiling of parental cells and gene-targeted derivatives identified numerous novel genes whose expression was dependent on the presence of oncogenic β-catenin. The transforming growth factor-β family member bone morphogenetic protein 4 (BMP4), whose receptor is mutated in a rare inherited gastrointestinal cancer predisposition syndrome, was the most highly differentially expressed gene. Additional experiments revealed that the oncogenic allele of β-catenin specifically is absolutely required for BMP4 expression and secretion by human cancer cells and that BMP4 is overexpressed and secreted by human colon cancer cells with mutant adenomatous polyposis coli genes. These data identify the presence of regulatory interactions between the Wnt and BMP signaling pathways in cancer pathogenesis, providing an intriguing connection between the sporadic and inherited forms of a common human malignancy.

Original languageEnglish (US)
Pages (from-to)2744-2748
Number of pages5
JournalCancer Research
Volume62
Issue number10
StatePublished - May 15 2002
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 4
Catenins
Neoplasms
Colonic Neoplasms
APC Genes
Bone Neoplasms
Wnt Signaling Pathway
Gastrointestinal Neoplasms
Gene Targeting
Transforming Growth Factors
Oncogenes
Genes
Alleles
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kim, J. S., Crooks, H., Dracheva, T., Nishanian, T. G., Singh, B., Jen, J., & Waldman, T. (2002). Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells. Cancer Research, 62(10), 2744-2748.

Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells. / Kim, Jung Sik; Crooks, Heather; Dracheva, Tatiana; Nishanian, Tagvor G.; Singh, Baljit; Jen, Jin; Waldman, Todd.

In: Cancer Research, Vol. 62, No. 10, 15.05.2002, p. 2744-2748.

Research output: Contribution to journalArticle

Kim, JS, Crooks, H, Dracheva, T, Nishanian, TG, Singh, B, Jen, J & Waldman, T 2002, 'Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells', Cancer Research, vol. 62, no. 10, pp. 2744-2748.
Kim JS, Crooks H, Dracheva T, Nishanian TG, Singh B, Jen J et al. Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells. Cancer Research. 2002 May 15;62(10):2744-2748.
Kim, Jung Sik ; Crooks, Heather ; Dracheva, Tatiana ; Nishanian, Tagvor G. ; Singh, Baljit ; Jen, Jin ; Waldman, Todd. / Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells. In: Cancer Research. 2002 ; Vol. 62, No. 10. pp. 2744-2748.
@article{85adaa0fc25842a885b27b640c717ae2,
title = "Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells",
abstract = "Somatic cell gene targeting was used to create an isogenic set of human colon cancer cells that differs only in the presence or absence of their endogenous activated β-catenin oncogene. Affymetrix Genechip expression profiling of parental cells and gene-targeted derivatives identified numerous novel genes whose expression was dependent on the presence of oncogenic β-catenin. The transforming growth factor-β family member bone morphogenetic protein 4 (BMP4), whose receptor is mutated in a rare inherited gastrointestinal cancer predisposition syndrome, was the most highly differentially expressed gene. Additional experiments revealed that the oncogenic allele of β-catenin specifically is absolutely required for BMP4 expression and secretion by human cancer cells and that BMP4 is overexpressed and secreted by human colon cancer cells with mutant adenomatous polyposis coli genes. These data identify the presence of regulatory interactions between the Wnt and BMP signaling pathways in cancer pathogenesis, providing an intriguing connection between the sporadic and inherited forms of a common human malignancy.",
author = "Kim, {Jung Sik} and Heather Crooks and Tatiana Dracheva and Nishanian, {Tagvor G.} and Baljit Singh and Jin Jen and Todd Waldman",
year = "2002",
month = "5",
day = "15",
language = "English (US)",
volume = "62",
pages = "2744--2748",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "10",

}

TY - JOUR

T1 - Oncogenic β-catenin is required for bone morphogenetic protein 4 expression in human cancer cells

AU - Kim, Jung Sik

AU - Crooks, Heather

AU - Dracheva, Tatiana

AU - Nishanian, Tagvor G.

AU - Singh, Baljit

AU - Jen, Jin

AU - Waldman, Todd

PY - 2002/5/15

Y1 - 2002/5/15

N2 - Somatic cell gene targeting was used to create an isogenic set of human colon cancer cells that differs only in the presence or absence of their endogenous activated β-catenin oncogene. Affymetrix Genechip expression profiling of parental cells and gene-targeted derivatives identified numerous novel genes whose expression was dependent on the presence of oncogenic β-catenin. The transforming growth factor-β family member bone morphogenetic protein 4 (BMP4), whose receptor is mutated in a rare inherited gastrointestinal cancer predisposition syndrome, was the most highly differentially expressed gene. Additional experiments revealed that the oncogenic allele of β-catenin specifically is absolutely required for BMP4 expression and secretion by human cancer cells and that BMP4 is overexpressed and secreted by human colon cancer cells with mutant adenomatous polyposis coli genes. These data identify the presence of regulatory interactions between the Wnt and BMP signaling pathways in cancer pathogenesis, providing an intriguing connection between the sporadic and inherited forms of a common human malignancy.

AB - Somatic cell gene targeting was used to create an isogenic set of human colon cancer cells that differs only in the presence or absence of their endogenous activated β-catenin oncogene. Affymetrix Genechip expression profiling of parental cells and gene-targeted derivatives identified numerous novel genes whose expression was dependent on the presence of oncogenic β-catenin. The transforming growth factor-β family member bone morphogenetic protein 4 (BMP4), whose receptor is mutated in a rare inherited gastrointestinal cancer predisposition syndrome, was the most highly differentially expressed gene. Additional experiments revealed that the oncogenic allele of β-catenin specifically is absolutely required for BMP4 expression and secretion by human cancer cells and that BMP4 is overexpressed and secreted by human colon cancer cells with mutant adenomatous polyposis coli genes. These data identify the presence of regulatory interactions between the Wnt and BMP signaling pathways in cancer pathogenesis, providing an intriguing connection between the sporadic and inherited forms of a common human malignancy.

UR - http://www.scopus.com/inward/record.url?scp=0037093072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037093072&partnerID=8YFLogxK

M3 - Article

C2 - 12019147

AN - SCOPUS:0037093072

VL - 62

SP - 2744

EP - 2748

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 10

ER -