On the operational characteristics of the Benjamini and Hochberg false discovery rate procedure

Gerwyn H. Green, Peter J. Diggle

Research output: Contribution to journalArticlepeer-review

Abstract

Multiple testing procedures are commonly used in gene expression studies for the detection of differential expression, where typically thousands of genes are measured over at least two experimental conditions. Given the need for powerful testing procedures, and the attendant danger of false positives in multiple testing, the False Discovery Rate (FDR) controlling procedure of Benjamini and Hochberg (1995) has become a popular tool. When simultaneously testing hypotheses, suppose that R rejections are made, of which Fp are false positives. The Benjamini and Hochberg procedure ensures that the expectation of Fp/R is bounded above by some pre-specified proportion. In practice, the procedure is applied to a single experiment. In this paper we investigate the across-experiment variability of the proportion Fp/R as a function of three experimental parameters. The operational characteristics of the procedure when applied to dependent hypotheses are also considered.

Original languageEnglish (US)
Article number27
JournalStatistical Applications in Genetics and Molecular Biology
Volume6
Issue number1
StatePublished - 2007
Externally publishedYes

Keywords

  • Differential expression
  • False discovery rate
  • Microarray
  • Multiple testing

ASJC Scopus subject areas

  • Genetics

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