On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes

L. W. Role, V. R. Matossian, R. J. O'Brien, G. D. Fischbach

Research output: Contribution to journalArticle

Abstract

We have examained the specificity and the mechanism of acetylcholine receptor (AChR) accumulation at embryonic chick nerve-muscle contacts that form in culture. Spinal cord motoneurons were identified in vitro after labelling them in vivo with Lucifer Yellow-wheat germ agglutinin conjugates. All of their processes induced receptor clusters on contacted myotubes; after 24 to 48 hr of co-culture, the incidence of neurite-associated receptor patches (NARPs) was ~ 1.2/100 μm of contact. In contrast, NARPs were rarely associated with spinal cord interneurons (~ 0.1/100 μm of contact). Neurons dissociated from ciliary ganglia induce NARPs to the same extent as motoneurons. The relative contribution to NARPs of AChRs present in the membrane prior to plating ciliary ganglion neurons and of 'new' AChRs inserted 8, 11, or 17 hr after addition of neurons was assessed with two fluorescent receptor probes. Rhodamine-conjugated α-bungarotoxin was used to label either old or new receptors; a monoclonal, anti-receptor antibody visualized with fluorescein-second antibody was used to label all (new and old) receptors. Analysis of digitized fluorescence images showed that NARPs contained both new and old receptors but that within the first 24 hr of co-culture the majority (60 to 80%) were new. We estimate that cholinergic neurites increase the rate of receptor insertion 4- to 5-fold during the first 8 hr of NARP formation. The contribution of new receptors to NARPs declines with time. After 3 days of co-culture, receptors inserted over an 8-hr interval comprised only 20% of the total NARP complement. Receptor clusters that appear beneath or adjacent to neuron cell-myotube contacts are apparently different from NARPs in that the contribution of newly inserted clusters never exceeded ~ 20% of the total even during the first 24 hr of co-culture. Thus, the relative importance for NARP formation of receptor migration within the membrane and of receptor insertion varies with time and position along the cell body-neurite axis.

Original languageEnglish (US)
Pages (from-to)2197-2204
Number of pages8
JournalJournal of Neuroscience
Volume5
Issue number8
StatePublished - 1985
Externally publishedYes

Fingerprint

Skeletal Muscle Fibers
Cholinergic Receptors
Neurites
Synapses
Coculture Techniques
Parasympathetic Ganglia
Neurons
Motor Neurons
Spinal Cord
Complement Receptors
Bungarotoxins
Wheat Germ Agglutinins
Rhodamines
Membranes
Interneurons
Fluorescein
Fluorescent Dyes
Cholinergic Agents
Anti-Idiotypic Antibodies
Fluorescence

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Role, L. W., Matossian, V. R., O'Brien, R. J., & Fischbach, G. D. (1985). On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes. Journal of Neuroscience, 5(8), 2197-2204.

On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes. / Role, L. W.; Matossian, V. R.; O'Brien, R. J.; Fischbach, G. D.

In: Journal of Neuroscience, Vol. 5, No. 8, 1985, p. 2197-2204.

Research output: Contribution to journalArticle

Role, LW, Matossian, VR, O'Brien, RJ & Fischbach, GD 1985, 'On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes', Journal of Neuroscience, vol. 5, no. 8, pp. 2197-2204.
Role, L. W. ; Matossian, V. R. ; O'Brien, R. J. ; Fischbach, G. D. / On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes. In: Journal of Neuroscience. 1985 ; Vol. 5, No. 8. pp. 2197-2204.
@article{42958c0379c74cf68e8096fac38b1a93,
title = "On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes",
abstract = "We have examained the specificity and the mechanism of acetylcholine receptor (AChR) accumulation at embryonic chick nerve-muscle contacts that form in culture. Spinal cord motoneurons were identified in vitro after labelling them in vivo with Lucifer Yellow-wheat germ agglutinin conjugates. All of their processes induced receptor clusters on contacted myotubes; after 24 to 48 hr of co-culture, the incidence of neurite-associated receptor patches (NARPs) was ~ 1.2/100 μm of contact. In contrast, NARPs were rarely associated with spinal cord interneurons (~ 0.1/100 μm of contact). Neurons dissociated from ciliary ganglia induce NARPs to the same extent as motoneurons. The relative contribution to NARPs of AChRs present in the membrane prior to plating ciliary ganglion neurons and of 'new' AChRs inserted 8, 11, or 17 hr after addition of neurons was assessed with two fluorescent receptor probes. Rhodamine-conjugated α-bungarotoxin was used to label either old or new receptors; a monoclonal, anti-receptor antibody visualized with fluorescein-second antibody was used to label all (new and old) receptors. Analysis of digitized fluorescence images showed that NARPs contained both new and old receptors but that within the first 24 hr of co-culture the majority (60 to 80{\%}) were new. We estimate that cholinergic neurites increase the rate of receptor insertion 4- to 5-fold during the first 8 hr of NARP formation. The contribution of new receptors to NARPs declines with time. After 3 days of co-culture, receptors inserted over an 8-hr interval comprised only 20{\%} of the total NARP complement. Receptor clusters that appear beneath or adjacent to neuron cell-myotube contacts are apparently different from NARPs in that the contribution of newly inserted clusters never exceeded ~ 20{\%} of the total even during the first 24 hr of co-culture. Thus, the relative importance for NARP formation of receptor migration within the membrane and of receptor insertion varies with time and position along the cell body-neurite axis.",
author = "Role, {L. W.} and Matossian, {V. R.} and O'Brien, {R. J.} and Fischbach, {G. D.}",
year = "1985",
language = "English (US)",
volume = "5",
pages = "2197--2204",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "8",

}

TY - JOUR

T1 - On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes

AU - Role, L. W.

AU - Matossian, V. R.

AU - O'Brien, R. J.

AU - Fischbach, G. D.

PY - 1985

Y1 - 1985

N2 - We have examained the specificity and the mechanism of acetylcholine receptor (AChR) accumulation at embryonic chick nerve-muscle contacts that form in culture. Spinal cord motoneurons were identified in vitro after labelling them in vivo with Lucifer Yellow-wheat germ agglutinin conjugates. All of their processes induced receptor clusters on contacted myotubes; after 24 to 48 hr of co-culture, the incidence of neurite-associated receptor patches (NARPs) was ~ 1.2/100 μm of contact. In contrast, NARPs were rarely associated with spinal cord interneurons (~ 0.1/100 μm of contact). Neurons dissociated from ciliary ganglia induce NARPs to the same extent as motoneurons. The relative contribution to NARPs of AChRs present in the membrane prior to plating ciliary ganglion neurons and of 'new' AChRs inserted 8, 11, or 17 hr after addition of neurons was assessed with two fluorescent receptor probes. Rhodamine-conjugated α-bungarotoxin was used to label either old or new receptors; a monoclonal, anti-receptor antibody visualized with fluorescein-second antibody was used to label all (new and old) receptors. Analysis of digitized fluorescence images showed that NARPs contained both new and old receptors but that within the first 24 hr of co-culture the majority (60 to 80%) were new. We estimate that cholinergic neurites increase the rate of receptor insertion 4- to 5-fold during the first 8 hr of NARP formation. The contribution of new receptors to NARPs declines with time. After 3 days of co-culture, receptors inserted over an 8-hr interval comprised only 20% of the total NARP complement. Receptor clusters that appear beneath or adjacent to neuron cell-myotube contacts are apparently different from NARPs in that the contribution of newly inserted clusters never exceeded ~ 20% of the total even during the first 24 hr of co-culture. Thus, the relative importance for NARP formation of receptor migration within the membrane and of receptor insertion varies with time and position along the cell body-neurite axis.

AB - We have examained the specificity and the mechanism of acetylcholine receptor (AChR) accumulation at embryonic chick nerve-muscle contacts that form in culture. Spinal cord motoneurons were identified in vitro after labelling them in vivo with Lucifer Yellow-wheat germ agglutinin conjugates. All of their processes induced receptor clusters on contacted myotubes; after 24 to 48 hr of co-culture, the incidence of neurite-associated receptor patches (NARPs) was ~ 1.2/100 μm of contact. In contrast, NARPs were rarely associated with spinal cord interneurons (~ 0.1/100 μm of contact). Neurons dissociated from ciliary ganglia induce NARPs to the same extent as motoneurons. The relative contribution to NARPs of AChRs present in the membrane prior to plating ciliary ganglion neurons and of 'new' AChRs inserted 8, 11, or 17 hr after addition of neurons was assessed with two fluorescent receptor probes. Rhodamine-conjugated α-bungarotoxin was used to label either old or new receptors; a monoclonal, anti-receptor antibody visualized with fluorescein-second antibody was used to label all (new and old) receptors. Analysis of digitized fluorescence images showed that NARPs contained both new and old receptors but that within the first 24 hr of co-culture the majority (60 to 80%) were new. We estimate that cholinergic neurites increase the rate of receptor insertion 4- to 5-fold during the first 8 hr of NARP formation. The contribution of new receptors to NARPs declines with time. After 3 days of co-culture, receptors inserted over an 8-hr interval comprised only 20% of the total NARP complement. Receptor clusters that appear beneath or adjacent to neuron cell-myotube contacts are apparently different from NARPs in that the contribution of newly inserted clusters never exceeded ~ 20% of the total even during the first 24 hr of co-culture. Thus, the relative importance for NARP formation of receptor migration within the membrane and of receptor insertion varies with time and position along the cell body-neurite axis.

UR - http://www.scopus.com/inward/record.url?scp=0022259157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022259157&partnerID=8YFLogxK

M3 - Article

C2 - 3839524

AN - SCOPUS:0022259157

VL - 5

SP - 2197

EP - 2204

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 8

ER -