On the equilibrium between monomeric α-lactalbumin and the chaperoning complex of α-crystallin

Rachel Neal, J. Samuel Zigler, Frederick A. Bettelheim

Research output: Contribution to journalArticle

Abstract

In chaperoning dithiothreitol-denatured α-lactabumin, α-crystallin forms a chaperoning complex. In order to study the kinetics of such chaperoning it needs to be established whether the formation of the chaperoning complex is a reversible or irreversible process. The chaperoning reaction was studied by dynamic light scattering as a function of concentration and weight ratio of α-lactalbumin/α-crystallin. HPLC and subsequent SDS-PAGE gel electrophoresis experiments established that the chaperoning complex formed contains both α-crystallin and α-lactalbumin. Upon rechromatographing the chaperoning complex, the presence of monomeric α-lactalbumin has been demonstrated in addition to the chaperoning complex itself. This and equilibrium dialysis experiments demonstrated conclusively the existence of an equilibrium between monomeric partially denatured α-lactalbumin and the chaperoning complex made of α-lactalbumin and α-crystallin.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Lactalbumin
Crystallins
Dialysis
Dithiothreitol
Dynamic light scattering
Electrophoresis
Polyacrylamide Gel Electrophoresis
Gels
Experiments
High Pressure Liquid Chromatography
Weights and Measures
Kinetics

Keywords

  • α-crystallin
  • α-lactalbumin
  • Chaperone
  • Equilibrium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

On the equilibrium between monomeric α-lactalbumin and the chaperoning complex of α-crystallin. / Neal, Rachel; Zigler, J. Samuel; Bettelheim, Frederick A.

In: Biochemical and Biophysical Research Communications, Vol. 280, No. 1, 2001, p. 14-18.

Research output: Contribution to journalArticle

Neal, Rachel ; Zigler, J. Samuel ; Bettelheim, Frederick A. / On the equilibrium between monomeric α-lactalbumin and the chaperoning complex of α-crystallin. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 280, No. 1. pp. 14-18.
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