On neglecting chemical exchange when correcting in vivo 31 P MRS data for partial saturation: Commentary on: "Pitfalls in the measurement of metabolite concentrations using the one-pulse experiment in in vivo NMR"

Ronald Ouwerkerk, Paul A. Bottomley

Research output: Contribution to journalEditorialpeer-review

Abstract

This article replies to Spencer et al. (J. Magn. Reson. 149, 251-257, 2001) concerning the degree to which chemical exchange affects partial saturation corrections using saturation factors. Considering the important case of in vivo 31 P NMR, we employ differential analysis to demonstrate a broad range of experimental conditions over which chemical exchange minimally affects saturation factors, and near-optimum signal-to-noise ratio is preserved. The analysis contradicts Spencer et al.'s broad claim that chemical exchange results in a strong dependence of saturation factors upon M 0 's and T 1 and exchange parameters. For Spencer et al.'s example of a dynamic 31 P NMR experiment in which phosphocreatine varies 20-fold, we show that our strategy of measuring saturation factors at the start and end of the study reduces errors in saturation corrections to 2% for the high-energy phosphates.

Original languageEnglish (US)
Pages (from-to)282-286
Number of pages5
JournalJournal of Magnetic Resonance
Volume149
Issue number2
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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