A method for the determination of the antitumor platinum(II) complex carboplatin has been developed that uses high-performance liquid chromatography with differential pulse polarographic (DPP) detection. Carboplatin is reduced at minus 1. 77 V vs. Ag/AgCl. DPP detection was used because of its sensitivity and selectivity. The influence of differential pulse polarographic parameters on detector performance was investigated. By use of a pulse amplitude of minus 250 mV at a potential of minus 1. 6 V, with a 0. 5-s drop time and a 50-ms memory time constant, detection limits of 0. 1 mu M carboplatin in plasma ultrafiltrate and 1 mu M in urine were achieved.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Sep 1986|
ASJC Scopus subject areas
- Analytical Chemistry