On-line differential pulse polarographic detection of carboplatin in biological samples after chromatographic separation

F. Elferink, W. J F Van Der Vijgh, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the determination of the antitumor platinum(II) complex carboplatin has been developed that uses high-performance liquid chromatography with differential pulse polarographic (DPP) detection. Carboplatin is reduced at minus 1. 77 V vs. Ag/AgCl. DPP detection was used because of its sensitivity and selectivity. The influence of differential pulse polarographic parameters on detector performance was investigated. By use of a pulse amplitude of minus 250 mV at a potential of minus 1. 6 V, with a 0. 5-s drop time and a 50-ms memory time constant, detection limits of 0. 1 mu M carboplatin in plasma ultrafiltrate and 1 mu M in urine were achieved.

Original languageEnglish (US)
Pages (from-to)2293-2296
Number of pages4
JournalAnalytical Chemistry
Volume58
Issue number11
StatePublished - Sep 1986
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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