Oligonucleoside methylphosphonates as antisense reagents

Paul S. Miller

Research output: Contribution to journalArticle


Oligonucleoside methylphosphonates contain nonionic internucleotide bonds that resist degradation by cellular nucleases and allow the oligomers to be taken up intact by mammalian cells in culture. Antisense methylphosphonate oligomers targeted against cellular or viral mRNA initation codon or coding regions or against precursor mRNA splice sites effectively and specifically inhibit mRNA expression in cells. The efficacy of antisense methylphosphonate oligomers can be enhanced by derivatization with functional groups that allow the oligomer to covalently cross-link with its targeted mRNA. These oligonucleotide analogs will be useful tools for studying and controlling gene expression and are also promising candidates for development as therapeutic agents.

Original languageEnglish (US)
Pages (from-to)358-362
Number of pages5
JournalNature Biotechnology
Issue number4
Publication statusPublished - 1991


ASJC Scopus subject areas

  • Microbiology

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