Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase

Wendy Xin, Yevgeniya A. Mironova, Hui Shen, Rosa A.M. Marino, Ari Waisman, Wouter H. Lamers, Dwight E Bergles, Antonello Bonci

Research output: Contribution to journalArticle

Abstract

Glutamate has been implicated in a wide range of brain pathologies and is thought to be metabolized via the astrocyte-specific enzyme glutamine synthetase (GS). We show here that oligodendrocytes, the myelinating glia of the central nervous system, also express high levels of GS in caudal regions like the midbrain and the spinal cord. Selective removal of oligodendrocyte GS in mice led to reduced brain glutamate and glutamine levels and impaired glutamatergic synaptic transmission without disrupting myelination. Furthermore, animals lacking oligodendrocyte GS displayed deficits in cocaine-induced locomotor sensitization, a behavior that is dependent on glutamatergic signaling in the midbrain. Thus, oligodendrocytes support glutamatergic transmission through the actions of GS and may represent a therapeutic target for pathological conditions related to brain glutamate dysregulation. Xin et al. show that mature oligodendrocytes, the myelinating cells of the brain, express the glutamine-synthesizing enzyme glutamine synthetase (GS). Oligodendrocyte-specific GS deletion does not impair myelination but disrupts neuronal glutamatergic transmission, thus demonstrating a myelin-independent role for oligodendrocytes in supporting glutamate signaling in the brain.

Original languageEnglish (US)
Pages (from-to)2262-2271.e5
JournalCell Reports
Volume27
Issue number8
DOIs
StatePublished - May 21 2019

Fingerprint

Glutamate-Ammonia Ligase
Oligodendroglia
Brain
Glutamic Acid
Mesencephalon
Glutamine
Neurology
Pathology
Enzymes
Myelin Sheath
Cocaine
Synaptic Transmission
Neuroglia
Astrocytes
Spinal Cord
Animals
Central Nervous System

Keywords

  • cocaine
  • glia
  • glutamate
  • glutamine
  • glutamine synthetase
  • midbrain
  • oligodendrocyte
  • transmission

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Xin, W., Mironova, Y. A., Shen, H., Marino, R. A. M., Waisman, A., Lamers, W. H., ... Bonci, A. (2019). Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase. Cell Reports, 27(8), 2262-2271.e5. https://doi.org/10.1016/j.celrep.2019.04.094

Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase. / Xin, Wendy; Mironova, Yevgeniya A.; Shen, Hui; Marino, Rosa A.M.; Waisman, Ari; Lamers, Wouter H.; Bergles, Dwight E; Bonci, Antonello.

In: Cell Reports, Vol. 27, No. 8, 21.05.2019, p. 2262-2271.e5.

Research output: Contribution to journalArticle

Xin, W, Mironova, YA, Shen, H, Marino, RAM, Waisman, A, Lamers, WH, Bergles, DE & Bonci, A 2019, 'Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase', Cell Reports, vol. 27, no. 8, pp. 2262-2271.e5. https://doi.org/10.1016/j.celrep.2019.04.094
Xin W, Mironova YA, Shen H, Marino RAM, Waisman A, Lamers WH et al. Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase. Cell Reports. 2019 May 21;27(8):2262-2271.e5. https://doi.org/10.1016/j.celrep.2019.04.094
Xin, Wendy ; Mironova, Yevgeniya A. ; Shen, Hui ; Marino, Rosa A.M. ; Waisman, Ari ; Lamers, Wouter H. ; Bergles, Dwight E ; Bonci, Antonello. / Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase. In: Cell Reports. 2019 ; Vol. 27, No. 8. pp. 2262-2271.e5.
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