Olig2-dependent developmental fate switch of NG2 cells

Xiaoqin Zhu, Hao Zuo, Brady Maher, David R. Serwanski, Joseph J. LoTurco, Q. Richard Lu, Akiko Nishiyama

Research output: Contribution to journalArticle

Abstract

NG2-expressing cells (NG2 cells or polydendrocytes) generate oligodendrocytes throughout the CNS and a subpopulation of protoplasmic astrocytes in the gray matter of the ventral forebrain. The mechanisms that regulate their oligodendrocyte or astrocyte fate and the degree to which they exhibit lineage plasticity in vivo have remained unclear. The basic helix-loop-helix transcription factor Olig2 is required for oligodendrocyte specification and differentiation. We have found that Olig2 expression is spontaneously downregulated in NG2 cells in the normal embryonic ventral forebrain as they differentiate into astrocytes. To further examine the role of Olig2 in NG2 cell fate determination, we used genetic fate mapping of NG2 cells in constitutive and tamoxifen-inducible Olig2 conditional knockout mice in which Olig2 was deleted specifically in NG2 cells. Constitutive deletion of Olig2 in NG2 cells in the neocortex and corpus callosum but not in ventral forebrain caused them to convert their fate into astrocytes, with a concomitant severe reduction in the number of oligodendrocytes and myelin. Deletion of Olig2 in NG2 cells in perinatal mice also resulted in astrocyte generation from neocortical NG2 cells. These observations indicate that the developmental fate of NG2 cells can be switched by altering a single transcription factor Olig2.

Original languageEnglish (US)
Pages (from-to)2299-2309
Number of pages11
JournalDevelopment (Cambridge)
Volume139
Issue number13
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

Fingerprint

Astrocytes
Oligodendroglia
Prosencephalon
Basic Helix-Loop-Helix Transcription Factors
Corpus Callosum
Neocortex
Tamoxifen
Myelin Sheath
Knockout Mice
Transcription Factors
Down-Regulation

Keywords

  • Astrocyte
  • Fate mapping
  • Myelin
  • NG2
  • Olig2
  • Oligodendrocyte progenitor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Zhu, X., Zuo, H., Maher, B., Serwanski, D. R., LoTurco, J. J., Richard Lu, Q., & Nishiyama, A. (2012). Olig2-dependent developmental fate switch of NG2 cells. Development (Cambridge), 139(13), 2299-2309. https://doi.org/10.1242/dev.078873

Olig2-dependent developmental fate switch of NG2 cells. / Zhu, Xiaoqin; Zuo, Hao; Maher, Brady; Serwanski, David R.; LoTurco, Joseph J.; Richard Lu, Q.; Nishiyama, Akiko.

In: Development (Cambridge), Vol. 139, No. 13, 01.07.2012, p. 2299-2309.

Research output: Contribution to journalArticle

Zhu, X, Zuo, H, Maher, B, Serwanski, DR, LoTurco, JJ, Richard Lu, Q & Nishiyama, A 2012, 'Olig2-dependent developmental fate switch of NG2 cells', Development (Cambridge), vol. 139, no. 13, pp. 2299-2309. https://doi.org/10.1242/dev.078873
Zhu X, Zuo H, Maher B, Serwanski DR, LoTurco JJ, Richard Lu Q et al. Olig2-dependent developmental fate switch of NG2 cells. Development (Cambridge). 2012 Jul 1;139(13):2299-2309. https://doi.org/10.1242/dev.078873
Zhu, Xiaoqin ; Zuo, Hao ; Maher, Brady ; Serwanski, David R. ; LoTurco, Joseph J. ; Richard Lu, Q. ; Nishiyama, Akiko. / Olig2-dependent developmental fate switch of NG2 cells. In: Development (Cambridge). 2012 ; Vol. 139, No. 13. pp. 2299-2309.
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