Olfactory neurons obtained through nasal biopsy combined with laser-capture microdissection: A potential approach to study treatment response in mental disorders

Soumya Narayan, Charlee McLean, Akira Sawa, Sandra Y. Lin, Narayan Rai, Maria Mananita S. Hipolito, Nicola Cascella, John J.I. Nurnberger, Koko Ishizuka, Evaristus A. Nwulia

Research output: Contribution to journalArticle

Abstract

Bipolar disorder (BD) is a severe neuropsychiatric disorder with poorly understood pathophysiology and typically treated with the mood stabilizer, lithium carbonate. Animal studies as well as human genetic studies indicate that lithium affects molecular targets that are involved in neuronal growth, survival and maturation, and notably molecules involved in Wnt signaling. Given the ethical challenge to obtaining brain biopsies for investigating dynamic molecular changes associated with lithium-response in the central nervous system (CNS), one may consider the use of neurons obtained from olfactory tissues to achieve this goal.The olfactory epithelium contains olfactory receptor neurons at different stages of development and glial-like supporting cells. This provides a unique opportunity to study dynamic changes in the CNS of patients with neuropsychiatric diseases, using olfactory tissue safely obtained from nasal biopsies. To overcome the drawback posed by substantial contamination of biopsied olfactory tissue with non-neuronal cells, a novel approach to obtain enriched neuronal cell populations was developed by combining nasal biopsies with laser-capture microdissection. In this study, a system for investigating treatment-associated dynamic molecular changes in neuronal tissue was developed and validated, using a small pilot sample of BD patients recruited for the study of the molecular mechanisms of lithium treatment response.

Original languageEnglish (US)
Article numbere51853
JournalJournal of Visualized Experiments
Issue number94
DOIs
StatePublished - Dec 4 2014

Keywords

  • Bipolar disorder
  • GSK-3β
  • Issue 94
  • Laser-capture microdissection
  • Lithium therapy
  • Nasal biopsy
  • Neuroscience
  • Olfactory epithelium
  • Real-time PCR

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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