Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice

Lin Li, Veronika Mauric, Jun Fang Zheng, SungUng Kang, Sudarshan Patil, Harald Höger, Gert Lubec

Research output: Contribution to journalArticle

Abstract

Information on systematic analysis of olfactory memory-related proteins is poor. In this study, the odor discrimination task to investigate olfactory recognition memory of adult male C57BL/6J mice was used. Subsequently, olfactory bulbs (OBs) were taken, proteins extracted, and run on two-dimensional gel electrophoresis with in-gel-protein digestion, followed by mass spectrom-etry and quantification of differentially expressed proteins. Dual specificity mitogen-activated protein kinase kinase 1 (MEK1), dihydropyrimidinase-related protein 1 (DRP1), and fascin are related with Lemon odor memory. Micro-tubule-associated protein RP/EB family member 3 is related to Rose odor memory. Hypoxanthine-guanine phosphoribosyltransferase is related with both Lemon and Rose odors memory. MEK1 and DRP1 levels were increased, while microtubule-associated protein RP/EB family member 3, fascin and hypoxanthine-guanine phosphoribosyltransferase levels were decreased during olfactory memory. In summary, neurogenesis, signal transduction, cytoskeleton, and nucleotide metabolism are involved in olfactory memory formation and storage of C57BL/6J mice.

Original languageEnglish (US)
Pages (from-to)871-886
Number of pages16
JournalAmino Acids
Volume39
Issue number3
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

Olfactory Bulb
Inbred C57BL Mouse
Data storage equipment
Odors
dihydropyrimidinase
Proteins
Hypoxanthine Phosphoribosyltransferase
Gels
MAP Kinase Kinase 1
Signal transduction
Microtubule-Associated Proteins
Neurogenesis
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Cytoskeleton
Metabolism
Proteolysis
Signal Transduction
Nucleotides
Odorants

Keywords

  • C57BL/6J mice
  • Olfactory bulb
  • Olfactory discrimination task
  • Olfactory memory

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Li, L., Mauric, V., Zheng, J. F., Kang, S., Patil, S., Höger, H., & Lubec, G. (2010). Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice. Amino Acids, 39(3), 871-886. https://doi.org/10.1007/s00726-010-0543-1

Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice. / Li, Lin; Mauric, Veronika; Zheng, Jun Fang; Kang, SungUng; Patil, Sudarshan; Höger, Harald; Lubec, Gert.

In: Amino Acids, Vol. 39, No. 3, 08.2010, p. 871-886.

Research output: Contribution to journalArticle

Li, L, Mauric, V, Zheng, JF, Kang, S, Patil, S, Höger, H & Lubec, G 2010, 'Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice', Amino Acids, vol. 39, no. 3, pp. 871-886. https://doi.org/10.1007/s00726-010-0543-1
Li, Lin ; Mauric, Veronika ; Zheng, Jun Fang ; Kang, SungUng ; Patil, Sudarshan ; Höger, Harald ; Lubec, Gert. / Olfactory bulb proteins linked to olfactory memory in C57BL/6J mice. In: Amino Acids. 2010 ; Vol. 39, No. 3. pp. 871-886.
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