Abstract
Information on systematic analysis of olfactory memory-related proteins is poor. In this study, the odor discrimination task to investigate olfactory recognition memory of adult male C57BL/6J mice was used. Subsequently, olfactory bulbs (OBs) were taken, proteins extracted, and run on two-dimensional gel electrophoresis with in-gel-protein digestion, followed by mass spectrom-etry and quantification of differentially expressed proteins. Dual specificity mitogen-activated protein kinase kinase 1 (MEK1), dihydropyrimidinase-related protein 1 (DRP1), and fascin are related with Lemon odor memory. Micro-tubule-associated protein RP/EB family member 3 is related to Rose odor memory. Hypoxanthine-guanine phosphoribosyltransferase is related with both Lemon and Rose odors memory. MEK1 and DRP1 levels were increased, while microtubule-associated protein RP/EB family member 3, fascin and hypoxanthine-guanine phosphoribosyltransferase levels were decreased during olfactory memory. In summary, neurogenesis, signal transduction, cytoskeleton, and nucleotide metabolism are involved in olfactory memory formation and storage of C57BL/6J mice.
Original language | English (US) |
---|---|
Pages (from-to) | 871-886 |
Number of pages | 16 |
Journal | Amino Acids |
Volume | 39 |
Issue number | 3 |
DOIs | |
State | Published - Aug 2010 |
Externally published | Yes |
Keywords
- C57BL/6J mice
- Olfactory bulb
- Olfactory discrimination task
- Olfactory memory
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry