OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing

François Busquet, Ruben Strecker, Jane M. Rawlings, Scott E. Belanger, Thomas Braunbeck, Gregory J. Carr, Peter Cenijn, Przemyslaw Fochtman, Anne Gourmelon, Nicole Hübler, Andre Kleensang, Melanie Knöbel, Carola Kussatz, Juliette Legler, Adam Lillicrap, Fernando Martínez-Jerónimo, Christian Polleichtner, Helena Rzodeczko, Edward Salinas, Katharina E. Schneider & 7 others Stefan Scholz, Evert Jan van den Brandhof, Leo T M van der Ven, Susanne Walter-Rohde, Stefan Weigt, Hilda Witters, Marlies Halder

Research output: Contribution to journalArticle

Abstract

A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.

Original languageEnglish (US)
Pages (from-to)496-511
Number of pages16
JournalRegulatory Toxicology and Pharmacology
Volume69
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Acute Toxicity Tests
Validation Studies
Zebrafish
Toxicity
Embryonic Structures
Testing
Fishes
Fish
Somites
Yolk Sac
Organisation for Economic Co-Operation and Development
Zygote
Poisons
Solubility
Tail
Coagulation
Guidelines

Keywords

  • Alternative method
  • Aquatic acute toxicity
  • Reproducibility
  • Validation
  • Zebrafish embryo

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. / Busquet, François; Strecker, Ruben; Rawlings, Jane M.; Belanger, Scott E.; Braunbeck, Thomas; Carr, Gregory J.; Cenijn, Peter; Fochtman, Przemyslaw; Gourmelon, Anne; Hübler, Nicole; Kleensang, Andre; Knöbel, Melanie; Kussatz, Carola; Legler, Juliette; Lillicrap, Adam; Martínez-Jerónimo, Fernando; Polleichtner, Christian; Rzodeczko, Helena; Salinas, Edward; Schneider, Katharina E.; Scholz, Stefan; van den Brandhof, Evert Jan; van der Ven, Leo T M; Walter-Rohde, Susanne; Weigt, Stefan; Witters, Hilda; Halder, Marlies.

In: Regulatory Toxicology and Pharmacology, Vol. 69, No. 3, 2014, p. 496-511.

Research output: Contribution to journalArticle

Busquet, F, Strecker, R, Rawlings, JM, Belanger, SE, Braunbeck, T, Carr, GJ, Cenijn, P, Fochtman, P, Gourmelon, A, Hübler, N, Kleensang, A, Knöbel, M, Kussatz, C, Legler, J, Lillicrap, A, Martínez-Jerónimo, F, Polleichtner, C, Rzodeczko, H, Salinas, E, Schneider, KE, Scholz, S, van den Brandhof, EJ, van der Ven, LTM, Walter-Rohde, S, Weigt, S, Witters, H & Halder, M 2014, 'OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing', Regulatory Toxicology and Pharmacology, vol. 69, no. 3, pp. 496-511. https://doi.org/10.1016/j.yrtph.2014.05.018
Busquet, François ; Strecker, Ruben ; Rawlings, Jane M. ; Belanger, Scott E. ; Braunbeck, Thomas ; Carr, Gregory J. ; Cenijn, Peter ; Fochtman, Przemyslaw ; Gourmelon, Anne ; Hübler, Nicole ; Kleensang, Andre ; Knöbel, Melanie ; Kussatz, Carola ; Legler, Juliette ; Lillicrap, Adam ; Martínez-Jerónimo, Fernando ; Polleichtner, Christian ; Rzodeczko, Helena ; Salinas, Edward ; Schneider, Katharina E. ; Scholz, Stefan ; van den Brandhof, Evert Jan ; van der Ven, Leo T M ; Walter-Rohde, Susanne ; Weigt, Stefan ; Witters, Hilda ; Halder, Marlies. / OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. In: Regulatory Toxicology and Pharmacology. 2014 ; Vol. 69, No. 3. pp. 496-511.
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AU - Busquet, François

AU - Strecker, Ruben

AU - Rawlings, Jane M.

AU - Belanger, Scott E.

AU - Braunbeck, Thomas

AU - Carr, Gregory J.

AU - Cenijn, Peter

AU - Fochtman, Przemyslaw

AU - Gourmelon, Anne

AU - Hübler, Nicole

AU - Kleensang, Andre

AU - Knöbel, Melanie

AU - Kussatz, Carola

AU - Legler, Juliette

AU - Lillicrap, Adam

AU - Martínez-Jerónimo, Fernando

AU - Polleichtner, Christian

AU - Rzodeczko, Helena

AU - Salinas, Edward

AU - Schneider, Katharina E.

AU - Scholz, Stefan

AU - van den Brandhof, Evert Jan

AU - van der Ven, Leo T M

AU - Walter-Rohde, Susanne

AU - Weigt, Stefan

AU - Witters, Hilda

AU - Halder, Marlies

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N2 - A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.

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KW - Alternative method

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KW - Validation

KW - Zebrafish embryo

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