Odorant-sensitive adenylate cyclase: Rapid, potent activation and desensitization in primary olfactory neuronal cultures

Gabriele V. Ronnett, Donna J. Parfitt, Lynda D Hester, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutylmethoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 μM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.

Original languageEnglish (US)
Pages (from-to)2366-2369
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number6
StatePublished - 1991

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Adenylyl Cyclases
Odorants
Psychologic Desensitization

Keywords

  • Cell culture
  • Olfaction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Odorant-sensitive adenylate cyclase: Rapid, potent activation and desensitization in primary olfactory neuronal cultures",
abstract = "Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutylmethoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 μM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular {"}memory{"} for odorants.",
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T1 - Odorant-sensitive adenylate cyclase

T2 - Rapid, potent activation and desensitization in primary olfactory neuronal cultures

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AU - Parfitt, Donna J.

AU - Hester, Lynda D

AU - Snyder, Solomon H

PY - 1991

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N2 - Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutylmethoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 μM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.

AB - Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutylmethoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 μM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.

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KW - Olfaction

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