Ocular fluorophotometry in the normal- and diabetic monkey

Carl W. Jones, Jose G. Cunha-Vaz, Ran Zeimer, Mark M. Rusin, Patricia W. Langenberg, Charles M. Vygantas, Mark O M Tso, Olga Jonasson

Research output: Contribution to journalArticle

Abstract

Normal- and diabetic rhesus monkeys without retinopathy demonstrable by ophthalmoscopy or fluorescein angiography were examined with ocular fluorophotometry to detect alterations in their blood-ocular barriers. All vitreous fluorophotometry values were corrected for fluorescence attributable to background levels and then normalized to a blood fluorescein level of 10 μg ml-1. Reproducibility studies demonstrated an average coefficient of variation of 0·17 for all animals combined. Insulin-dependent monkeys, both pancreatectomized and strepozotocin-treated, demonstrated significantly higher posterior vitreous fluorescence levels than either control animals or monkeys treated with streptozotocin that were not insulin-dependent. These results cannot be attributed to differences in fluorescein binding or to vitreous abnormalities. However, 14 out of 24 (58%) of the insulin-dependent animals exhibited posterior vitreous fluorescence values within two standard deviations of the control mean. No correlation was apparent between the vitreous values and age or duration of treatment. No difference in anterior chamber concentrations was found between groups after correction. Our results indicate that alterations in the blood-retinal barrier can occur in insulin-dependent diabetic monkeys before development of retinopathy.

Original languageEnglish (US)
Pages (from-to)467-477
Number of pages11
JournalExperimental Eye Research
Volume42
Issue number5
DOIs
StatePublished - 1986

Fingerprint

Fluorophotometry
Haplorhini
Insulin
Fluorescence
Fluorescein
Blood-Retinal Barrier
Ophthalmoscopy
Fluorescein Angiography
Anterior Chamber
Streptozocin
Macaca mulatta

Keywords

  • blood-retinal barrier
  • diabetes
  • monkey
  • ocular fluorophotometry
  • permeability

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Jones, C. W., Cunha-Vaz, J. G., Zeimer, R., Rusin, M. M., Langenberg, P. W., Vygantas, C. M., ... Jonasson, O. (1986). Ocular fluorophotometry in the normal- and diabetic monkey. Experimental Eye Research, 42(5), 467-477. https://doi.org/10.1016/0014-4835(86)90006-0

Ocular fluorophotometry in the normal- and diabetic monkey. / Jones, Carl W.; Cunha-Vaz, Jose G.; Zeimer, Ran; Rusin, Mark M.; Langenberg, Patricia W.; Vygantas, Charles M.; Tso, Mark O M; Jonasson, Olga.

In: Experimental Eye Research, Vol. 42, No. 5, 1986, p. 467-477.

Research output: Contribution to journalArticle

Jones, CW, Cunha-Vaz, JG, Zeimer, R, Rusin, MM, Langenberg, PW, Vygantas, CM, Tso, MOM & Jonasson, O 1986, 'Ocular fluorophotometry in the normal- and diabetic monkey', Experimental Eye Research, vol. 42, no. 5, pp. 467-477. https://doi.org/10.1016/0014-4835(86)90006-0
Jones CW, Cunha-Vaz JG, Zeimer R, Rusin MM, Langenberg PW, Vygantas CM et al. Ocular fluorophotometry in the normal- and diabetic monkey. Experimental Eye Research. 1986;42(5):467-477. https://doi.org/10.1016/0014-4835(86)90006-0
Jones, Carl W. ; Cunha-Vaz, Jose G. ; Zeimer, Ran ; Rusin, Mark M. ; Langenberg, Patricia W. ; Vygantas, Charles M. ; Tso, Mark O M ; Jonasson, Olga. / Ocular fluorophotometry in the normal- and diabetic monkey. In: Experimental Eye Research. 1986 ; Vol. 42, No. 5. pp. 467-477.
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