Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV

T. R. Dinesha, J. Boobalan, S. Sivamalar, D. Subashini, Sunil Solomon, K. G. Murugavel, P. Balakrishnan, D. M. Smith, S. Saravanan

Research output: Contribution to journalArticle

Abstract

The study aimed to determine the prevalence of occult hepatitis B virus infection among HIV-infected persons and to evaluate the use of a pooling strategy to detect occult HBV infection in the setting of HIV infection. Five hundred and two HIV-positive individuals were tested for HBV, occult HBV and hepatitis C and D with serologic and nucleic acid testing (NAT). We also evaluated a pooled NAT strategy for screening occult HBV infection among the HIV-positive individuals. The prevalence of HBV infection among HIV-positive individuals was 32 (6.4%), and occult HBV prevalence was 10%. The pooling HBV NAT had a sensitivity of 66.7% and specificity of 100%, compared to HBV DNA NAT of individual samples. In conclusion, this study found a high prevalence of occult HBV infection among our HIV-infected population. We also demonstrated that pooled HBV NAT is highly specific, moderately sensitive and cost-effective. As conventional HBV viral load assays are expensive in resource-limited settings such as India, pooled HBV DNA NAT might be a good way for detecting occult HBV infection and will reduce HBV-associated complications.

Original languageEnglish (US)
Pages (from-to)718-723
Number of pages6
JournalJournal of Viral Hepatitis
Volume25
Issue number6
DOIs
StatePublished - Jun 1 2018

Fingerprint

Nucleic Acids
HIV Infections
HIV
Infection
Hepatitis D
DNA
Virus Diseases
Hepatitis C
Viral Load
Hepatitis B virus
India
Costs and Cost Analysis
Sensitivity and Specificity
Population

Keywords

  • HBV
  • HIV
  • occult HBV
  • pooled NAT and resource-limited settings

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases
  • Virology

Cite this

Dinesha, T. R., Boobalan, J., Sivamalar, S., Subashini, D., Solomon, S., Murugavel, K. G., ... Saravanan, S. (2018). Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV. Journal of Viral Hepatitis, 25(6), 718-723. https://doi.org/10.1111/jvh.12858

Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV. / Dinesha, T. R.; Boobalan, J.; Sivamalar, S.; Subashini, D.; Solomon, Sunil; Murugavel, K. G.; Balakrishnan, P.; Smith, D. M.; Saravanan, S.

In: Journal of Viral Hepatitis, Vol. 25, No. 6, 01.06.2018, p. 718-723.

Research output: Contribution to journalArticle

Dinesha, TR, Boobalan, J, Sivamalar, S, Subashini, D, Solomon, S, Murugavel, KG, Balakrishnan, P, Smith, DM & Saravanan, S 2018, 'Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV', Journal of Viral Hepatitis, vol. 25, no. 6, pp. 718-723. https://doi.org/10.1111/jvh.12858
Dinesha TR, Boobalan J, Sivamalar S, Subashini D, Solomon S, Murugavel KG et al. Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV. Journal of Viral Hepatitis. 2018 Jun 1;25(6):718-723. https://doi.org/10.1111/jvh.12858
Dinesha, T. R. ; Boobalan, J. ; Sivamalar, S. ; Subashini, D. ; Solomon, Sunil ; Murugavel, K. G. ; Balakrishnan, P. ; Smith, D. M. ; Saravanan, S. / Occult HBV infection in HIV-infected adults and evaluation of pooled NAT for HBV. In: Journal of Viral Hepatitis. 2018 ; Vol. 25, No. 6. pp. 718-723.
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