O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits

Lance Wells, Lisa K. Kreppel, Frank I. Comer, Brian E. Wadzinski, Gerald Warren Hart

Research output: Contribution to journalArticle

Abstract

A hallmark of signal transduction is the dynamic and inducible post-translational modification of proteins. In addition to the well characterized phosphorylation of proteins, other modifications have been shown to be regulatory, including O-linked β-N-acetylglucosamine (O-GlcNAc). O-GlcNAc modifies serine and threonine residues on a myriad of nuclear and cytosolic proteins, and for several proteins there appears to be a reciprocal relationship between phosphorylation and O-GlcNAc modification. Here we report further evidence of this yin-yang relationship by demonstrating that O-GlcNAc transferase, the enzyme that adds O-GlcNAc to proteins, exists in stable and active complexes with the serine/tlireonine phosphatases PP1β and PP1γ, enzymes that remove phosphate from proteins. The existence of this complex highlights the importance of understanding the dynamic relationship between O-GlcNAc and phosphate in modulating protein function in many cellular processes and disease states such as Alzheimer's disease and type II diabetes.

Original languageEnglish (US)
Pages (from-to)38466-38470
Number of pages5
JournalJournal of Biological Chemistry
Volume279
Issue number37
DOIs
StatePublished - Sep 10 2004

Fingerprint

Protein Phosphatase 1
Proteins
Serine
Phosphorylation
Phosphates
Yin-Yang
Acetylglucosamine
Enzymes
Threonine
Post Translational Protein Processing
Nuclear Proteins
Phosphoric Monoester Hydrolases
Type 2 Diabetes Mellitus
Signal transduction
Signal Transduction
Alzheimer Disease
Medical problems
O-GlcNAc transferase

ASJC Scopus subject areas

  • Biochemistry

Cite this

O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits. / Wells, Lance; Kreppel, Lisa K.; Comer, Frank I.; Wadzinski, Brian E.; Hart, Gerald Warren.

In: Journal of Biological Chemistry, Vol. 279, No. 37, 10.09.2004, p. 38466-38470.

Research output: Contribution to journalArticle

Wells, Lance ; Kreppel, Lisa K. ; Comer, Frank I. ; Wadzinski, Brian E. ; Hart, Gerald Warren. / O-GlcNAc transferase is in a functional complex with protein phosphatase 1 catalytic subunits. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 37. pp. 38466-38470.
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