Nutraceutical with resveratrol and omega-3 fatty acids induces autophagy in ARPE-19 cells

Ali Koskela, Mika Reinisalo, Goran Petrovski, Debasish Sinha, Céline Olmiere, Reijo Karjalainen, Kai Kaarniranta

Research output: Contribution to journalArticlepeer-review

Abstract

Impaired autophagic and proteasomal cleansing have been documented in aged retinal pigment epithelial (RPE) cells and age-related macular degeneration (AMD). Omega-3 fatty acids and resveratrol have many positive homeostatic effects in RPE cells. In this work, ARPE-19 cells were treated with 288 ng of Resvega, containing 30 mg of trans resveratrol and 665 mg of omega-3 fatty acids, among other nutrients, with proteasome inhibitor MG-132 or autophagy inhibitor bafilomycin A1 up to 48 h. Autophagy markers p62/SQSTM1 (p62) and LC3 (microtubule-associated protein 1A/1B-light chain 3) were analyzed by Western blotting. Fluorescence microscopy with mCherry-GFP-LC3 plasmid was applied to study the autophagy flux, and cytoprotective effects were investigated with colorimetric MTT and LDH assays. Resvega induced autophagy by showing increased autolysosome formation and autophagy flux, and the change in the p62 and LC3 protein levels further confirmed the fluorescent microscopy results. Moreover, Resvega provided a clear cytoprotection under proteasome inhibition. These findings highlight the potential of the nutraceuticals containing resveratrol, omega-3 fatty acids and other nutrients in the prevention of ARPE-19 cell damage.

Original languageEnglish (US)
Article number284
JournalNutrients
Volume8
Issue number5
DOIs
StatePublished - May 11 2016

Keywords

  • Autophagy
  • LC3
  • Omega-3
  • P62/SQSTM1
  • Proteasome
  • Resveratrol

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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