Nucleotide sequence of hamster spermidine/spermine-N1-acetyltransferase cDNA

Anthony E. Pegg, Bruce A. Stanley, Laurie Wiest, Robert A. Casero

Research output: Contribution to journalArticle

Abstract

The nucleotide sequence of a cDNA encoding hamster spermidine/spermine-N1-acetyltransferase, a key enzyme in polyamine degradation and excretion, has been determined. The cDNA consists of a 1016 base pair insert including 120 nucleotides of the 5′ untranslated region and the complete 3′ untranslated region. The deduced amino acid sequence is very similar to the human spermidine/spermine-N1-acetyltransferase with only 8 differences in 171 amino acids and the corresponding nucleotide sequence shows 91% identity. The 5′ untranslated regions are even more closely related with 97% identity suggesting that this region may play a role in the regulation of acetyltransferase activity. Translation of the acetyltransferase mRNA in a reticulocyte lysate was not altered by the addition of N1,N12-bis(ethyl)spermine.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1171
Issue number1
DOIs
StatePublished - Nov 15 1992

Keywords

  • (Hamster)
  • BESM
  • Polyamine metabolism
  • SAT
  • cDNA sequence

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'Nucleotide sequence of hamster spermidine/spermine-N<sup>1</sup>-acetyltransferase cDNA'. Together they form a unique fingerprint.

  • Cite this