Nucleotide sequence of hamster spermidine/spermine-N1-acetyltransferase cDNA

Anthony E. Pegg, Bruce A. Stanley, Laurie Wiest, Robert A Casero

Research output: Contribution to journalArticle

Abstract

The nucleotide sequence of a cDNA encoding hamster spermidine/spermine-N1-acetyltransferase, a key enzyme in polyamine degradation and excretion, has been determined. The cDNA consists of a 1016 base pair insert including 120 nucleotides of the 5′ untranslated region and the complete 3′ untranslated region. The deduced amino acid sequence is very similar to the human spermidine/spermine-N1-acetyltransferase with only 8 differences in 171 amino acids and the corresponding nucleotide sequence shows 91% identity. The 5′ untranslated regions are even more closely related with 97% identity suggesting that this region may play a role in the regulation of acetyltransferase activity. Translation of the acetyltransferase mRNA in a reticulocyte lysate was not altered by the addition of N1,N12-bis(ethyl)spermine.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1171
Issue number1
DOIs
StatePublished - Nov 15 1992

Fingerprint

Acetyltransferases
Spermidine
Spermine
Cricetinae
Nucleotides
Complementary DNA
5' Untranslated Regions
Reticulocytes
Polyamines
Protein Biosynthesis
3' Untranslated Regions
Amino Acids
Base Pairing
Amino Acid Sequence
Enzymes
Degradation
Messenger RNA
diamine N-acetyltransferase

Keywords

  • (Hamster)
  • BESM
  • cDNA sequence
  • Polyamine metabolism
  • SAT

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Nucleotide sequence of hamster spermidine/spermine-N1-acetyltransferase cDNA. / Pegg, Anthony E.; Stanley, Bruce A.; Wiest, Laurie; Casero, Robert A.

In: BBA - Gene Structure and Expression, Vol. 1171, No. 1, 15.11.1992, p. 106-108.

Research output: Contribution to journalArticle

Pegg, Anthony E. ; Stanley, Bruce A. ; Wiest, Laurie ; Casero, Robert A. / Nucleotide sequence of hamster spermidine/spermine-N1-acetyltransferase cDNA. In: BBA - Gene Structure and Expression. 1992 ; Vol. 1171, No. 1. pp. 106-108.
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