Abstract
The nucleotide sequence of a cDNA encoding hamster spermidine/spermine-N1-acetyltransferase, a key enzyme in polyamine degradation and excretion, has been determined. The cDNA consists of a 1016 base pair insert including 120 nucleotides of the 5′ untranslated region and the complete 3′ untranslated region. The deduced amino acid sequence is very similar to the human spermidine/spermine-N1-acetyltransferase with only 8 differences in 171 amino acids and the corresponding nucleotide sequence shows 91% identity. The 5′ untranslated regions are even more closely related with 97% identity suggesting that this region may play a role in the regulation of acetyltransferase activity. Translation of the acetyltransferase mRNA in a reticulocyte lysate was not altered by the addition of N1,N12-bis(ethyl)spermine.
Original language | English (US) |
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Pages (from-to) | 106-108 |
Number of pages | 3 |
Journal | BBA - Gene Structure and Expression |
Volume | 1171 |
Issue number | 1 |
DOIs | |
State | Published - Nov 15 1992 |
Keywords
- (Hamster)
- BESM
- Polyamine metabolism
- SAT
- cDNA sequence
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics