Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1

Robert C. Benjamin, Wayne P. Fitzmaurice, P C Huang, John J. Scocca

Research output: Contribution to journalArticle

Abstract

Restriction fragments obtained by digestion of Haemophilus influenzae phage HP1c1 DNA with HaeIII have been cloned by insertion into the HindIII site of pBR322 using synthetic linkers. The nucleotide sequences have been determined for three adjacent fragments, HaeIII-E, HaeIII-C and HaeIII-K, which comprise an 8.2-kb segment of the HP1c1 genome. The distribution and location of restriction sites in the sequenced region were determined. Restriction sites containing the dinucleotides -GG- and -CC- occurred infrequently in the sequence. The region contains numerous sequences which are subsets of the high-affinity recognition sequence of Haemophilus transformation, including five sites which direct high-affinity uptake of fragments containing them. Shorter subsets of the uptake sequence, including those with little or no measurable affinity for the DNA transport system, are considerably over-represented in HP1c1 DNA. Nine open reading frames (ORFs) corresponding to presumed polypeptides longer than 90 amino acid residues were identified; all of these shared a common orientation, suggesting the probable direction of transcription in this segment of the phage genome. These ORFs were preceded by appropriately spaced polypurine stretches which might function as ribosome-binding sites. One ORF coincides with the site of a mutation affecting the production of phage tails.

Original languageEnglish (US)
Pages (from-to)173-185
Number of pages13
JournalGene
Volume31
Issue number1-3
DOIs
StatePublished - 1984

Fingerprint

Haemophilus influenzae
Bacteriophages
Open Reading Frames
DNA
Genome
Haemophilus
Ribosomes
Digestion
Binding Sites
Amino Acids
Peptides
Mutation

Keywords

  • coliphage λ
  • DNA recognition sites for bacterial transformation
  • lysogenic phage
  • Maxam-Gilbert sequencing
  • open reading frames
  • recombinant DNA plasmids
  • restriction sites

ASJC Scopus subject areas

  • Genetics

Cite this

Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1. / Benjamin, Robert C.; Fitzmaurice, Wayne P.; Huang, P C; Scocca, John J.

In: Gene, Vol. 31, No. 1-3, 1984, p. 173-185.

Research output: Contribution to journalArticle

Benjamin, Robert C. ; Fitzmaurice, Wayne P. ; Huang, P C ; Scocca, John J. / Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1. In: Gene. 1984 ; Vol. 31, No. 1-3. pp. 173-185.
@article{0c11286ebb9d4c92a787ceb5baf85a8b,
title = "Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1",
abstract = "Restriction fragments obtained by digestion of Haemophilus influenzae phage HP1c1 DNA with HaeIII have been cloned by insertion into the HindIII site of pBR322 using synthetic linkers. The nucleotide sequences have been determined for three adjacent fragments, HaeIII-E, HaeIII-C and HaeIII-K, which comprise an 8.2-kb segment of the HP1c1 genome. The distribution and location of restriction sites in the sequenced region were determined. Restriction sites containing the dinucleotides -GG- and -CC- occurred infrequently in the sequence. The region contains numerous sequences which are subsets of the high-affinity recognition sequence of Haemophilus transformation, including five sites which direct high-affinity uptake of fragments containing them. Shorter subsets of the uptake sequence, including those with little or no measurable affinity for the DNA transport system, are considerably over-represented in HP1c1 DNA. Nine open reading frames (ORFs) corresponding to presumed polypeptides longer than 90 amino acid residues were identified; all of these shared a common orientation, suggesting the probable direction of transcription in this segment of the phage genome. These ORFs were preceded by appropriately spaced polypurine stretches which might function as ribosome-binding sites. One ORF coincides with the site of a mutation affecting the production of phage tails.",
keywords = "coliphage λ, DNA recognition sites for bacterial transformation, lysogenic phage, Maxam-Gilbert sequencing, open reading frames, recombinant DNA plasmids, restriction sites",
author = "Benjamin, {Robert C.} and Fitzmaurice, {Wayne P.} and Huang, {P C} and Scocca, {John J.}",
year = "1984",
doi = "10.1016/0378-1119(84)90208-7",
language = "English (US)",
volume = "31",
pages = "173--185",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1

AU - Benjamin, Robert C.

AU - Fitzmaurice, Wayne P.

AU - Huang, P C

AU - Scocca, John J.

PY - 1984

Y1 - 1984

N2 - Restriction fragments obtained by digestion of Haemophilus influenzae phage HP1c1 DNA with HaeIII have been cloned by insertion into the HindIII site of pBR322 using synthetic linkers. The nucleotide sequences have been determined for three adjacent fragments, HaeIII-E, HaeIII-C and HaeIII-K, which comprise an 8.2-kb segment of the HP1c1 genome. The distribution and location of restriction sites in the sequenced region were determined. Restriction sites containing the dinucleotides -GG- and -CC- occurred infrequently in the sequence. The region contains numerous sequences which are subsets of the high-affinity recognition sequence of Haemophilus transformation, including five sites which direct high-affinity uptake of fragments containing them. Shorter subsets of the uptake sequence, including those with little or no measurable affinity for the DNA transport system, are considerably over-represented in HP1c1 DNA. Nine open reading frames (ORFs) corresponding to presumed polypeptides longer than 90 amino acid residues were identified; all of these shared a common orientation, suggesting the probable direction of transcription in this segment of the phage genome. These ORFs were preceded by appropriately spaced polypurine stretches which might function as ribosome-binding sites. One ORF coincides with the site of a mutation affecting the production of phage tails.

AB - Restriction fragments obtained by digestion of Haemophilus influenzae phage HP1c1 DNA with HaeIII have been cloned by insertion into the HindIII site of pBR322 using synthetic linkers. The nucleotide sequences have been determined for three adjacent fragments, HaeIII-E, HaeIII-C and HaeIII-K, which comprise an 8.2-kb segment of the HP1c1 genome. The distribution and location of restriction sites in the sequenced region were determined. Restriction sites containing the dinucleotides -GG- and -CC- occurred infrequently in the sequence. The region contains numerous sequences which are subsets of the high-affinity recognition sequence of Haemophilus transformation, including five sites which direct high-affinity uptake of fragments containing them. Shorter subsets of the uptake sequence, including those with little or no measurable affinity for the DNA transport system, are considerably over-represented in HP1c1 DNA. Nine open reading frames (ORFs) corresponding to presumed polypeptides longer than 90 amino acid residues were identified; all of these shared a common orientation, suggesting the probable direction of transcription in this segment of the phage genome. These ORFs were preceded by appropriately spaced polypurine stretches which might function as ribosome-binding sites. One ORF coincides with the site of a mutation affecting the production of phage tails.

KW - coliphage λ

KW - DNA recognition sites for bacterial transformation

KW - lysogenic phage

KW - Maxam-Gilbert sequencing

KW - open reading frames

KW - recombinant DNA plasmids

KW - restriction sites

UR - http://www.scopus.com/inward/record.url?scp=0021721850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021721850&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(84)90208-7

DO - 10.1016/0378-1119(84)90208-7

M3 - Article

C2 - 6098523

AN - SCOPUS:0021721850

VL - 31

SP - 173

EP - 185

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-3

ER -