Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion

Chitrita Debroy, Nels Pederson, Stanley Person

Research output: Contribution to journalArticle

Abstract

The nucleotide sequence (2041 nucleotides) of a genomic region of herpes simplex virus type 1 (KOS strain) associated with virus-induced cell fusion has been determined. The sequence is bounded by a NruI site at 0.732 and a BamHI site at 0.745 prototypic map units. An open reading frame in the left-to-right orientation specifies a protein of 338 amino acids. The protein is positively charged. Since secondary structure analysis predicts four extensive hydrophobic domains the protein is probably a membrane associated or a transmembrane protein. Transcription of the putative fusion gene is dependent on viral DNA synthesis, characteristic of the late (γ) viral gene class. Two syncytia-inducing mutations, syn20 and MP, have been previously mapped to a 504-base pair PstI fragment within these genomic coordinates (V. C. Bond and S. Person (1984), Virology 132, 368-376). The nucleotide sequence of the PstI fragment was determined for the two mutants. Both were shown to have an amino acid substitution at residue 40 of the fusion protein. A second change at residue 101 for MP is probably unrelated to the fusion phenotype.

Original languageEnglish (US)
Pages (from-to)36-48
Number of pages13
JournalVirology
Volume145
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Cell Fusion
Human Herpesvirus 1
Genes
Proteins
Satellite Viruses
Virology
Viral Genes
Gene Fusion
Viral DNA
Giant Cells
Amino Acid Substitution
Base Pairing
Open Reading Frames
Nucleotides
Phenotype
Amino Acids
Mutation
Membranes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion. / Debroy, Chitrita; Pederson, Nels; Person, Stanley.

In: Virology, Vol. 145, No. 1, 1985, p. 36-48.

Research output: Contribution to journalArticle

Debroy, Chitrita ; Pederson, Nels ; Person, Stanley. / Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion. In: Virology. 1985 ; Vol. 145, No. 1. pp. 36-48.
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