Nucleotide sequence and properties of the cohesive DNA termini from bacteriophage HP1c1 of Haemophilus influenzae Rd

Wayne P. Fitzmaurice, Alan S. Waldman, Robert C. Benjamin, P. C. Huang, John J. Scocca

Research output: Contribution to journalArticle

Abstract

The termini of the mature DNA of phage HP1c1 of Haemophilus influenzae Rd have been characterized by DNA ligation, nucleotide sequencing, and deoxynucleotide incorporation experiments. A hybrid plasmid containing the joined phage termini (the cos site) inserted into pBR322 has been constructed. The phage DNA has cohesive termini composed of complementary 5 ' single-stranded extensions which are seven residues long. The left cohesive terminal extension consists only of pyrimidines and the right only of purines. When the ends of the phage are joined, the terminal sequences constitute the central 7 bp of an 11 bp sequence containing only purines on one strand and pyrimidines on the other strand. This oligopyrimidine/oligopurine sequence does not possess rotational symmetry. A 10-bp sequence and its inverted repeat are located approx. 20 bp to the left and right of the fused ends.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalGene
Volume31
Issue number1-3
DOIs
StatePublished - Nov 1984

Keywords

  • Maxam-Gilbert sequencing
  • lysogenic phage
  • oligopurine/oligopyrimidine clusters
  • recombinant plasmids
  • λ cos site

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Nucleotide sequence and properties of the cohesive DNA termini from bacteriophage HP1c1 of Haemophilus influenzae Rd'. Together they form a unique fingerprint.

  • Cite this