Nucleotide oligomerization domain 2 polymorphisms in patients with intestinal failure

Juan Francisco Guerra, Michael Zasloff, Denver Lough, Joseph Abdo, Jason Hawksworth, Cal Mastumoto, Raffaele Girlanda, Eddie Island, Kirty Shetty, Stuart Kaufman, Thomas Fishbein

Research output: Contribution to journalArticle

Abstract

Background: Nucleotide oligomerization domain 2 (NOD2) has been associated with intestinal immunity after the discovery that its polymorphisms are linked to Crohn's disease (CD). Intestinal failure (IF) represents a wider spectrum of diseases where intestinal homeostasis has been disrupted. Aim: To evaluate the prevalence of NOD2 mutations in a population with IF as well as its association with the different conditions causing this problem. Methods: One hundred ninety-two consecutive patients with IF and 103 healthy controls were genotyped for the three most common NOD2 polymorphisms. Genotypes were compared between the groups and were related to the entities causing IF. Results: A high percentage (26%) of patients had at least one of the three most common NOD2 polymorphisms, while only a 4.8% of healthy controls had a mutant genotype. In patients with IF, specific mutations for the 702W, 908R and 1007fs alleles were 11, 5 and 12.5%, respectively, compared with 0.9% (P=0.0003), 1.9% (P=0.1) and 1.9% (P=0.001) in the control group. If we consider patients with any cause of IF other than CD, the percentage is still as high as 18.8%, with specific mutation frequencies of 7.6% (702W; P=0.01), 5.8% (908R; P=0.1) and 8.2% (1007fs; P=0.002). We could not establish an association between a NOD2 mutant genotype with any other specific clinical condition other than CD. Conclusion: Our finding supports the importance of NOD2 in the maintenance of intestinal immune homeostasis and may be important to a variety of intestinal stressors.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume28
Issue number2
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Nucleotides
Crohn Disease
Genotype
Homeostasis
Intestinal Diseases
Mutation
Mutation Rate
Immunity
Alleles
Maintenance
Control Groups
Population

Keywords

  • Innate immunity
  • Intestinal failure
  • Intestinal homeostasis
  • NOD2 polymorphisms

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Guerra, J. F., Zasloff, M., Lough, D., Abdo, J., Hawksworth, J., Mastumoto, C., ... Fishbein, T. (2013). Nucleotide oligomerization domain 2 polymorphisms in patients with intestinal failure. Journal of Gastroenterology and Hepatology (Australia), 28(2), 309-313. https://doi.org/10.1111/jgh.12037

Nucleotide oligomerization domain 2 polymorphisms in patients with intestinal failure. / Guerra, Juan Francisco; Zasloff, Michael; Lough, Denver; Abdo, Joseph; Hawksworth, Jason; Mastumoto, Cal; Girlanda, Raffaele; Island, Eddie; Shetty, Kirty; Kaufman, Stuart; Fishbein, Thomas.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 28, No. 2, 2013, p. 309-313.

Research output: Contribution to journalArticle

Guerra, JF, Zasloff, M, Lough, D, Abdo, J, Hawksworth, J, Mastumoto, C, Girlanda, R, Island, E, Shetty, K, Kaufman, S & Fishbein, T 2013, 'Nucleotide oligomerization domain 2 polymorphisms in patients with intestinal failure', Journal of Gastroenterology and Hepatology (Australia), vol. 28, no. 2, pp. 309-313. https://doi.org/10.1111/jgh.12037
Guerra, Juan Francisco ; Zasloff, Michael ; Lough, Denver ; Abdo, Joseph ; Hawksworth, Jason ; Mastumoto, Cal ; Girlanda, Raffaele ; Island, Eddie ; Shetty, Kirty ; Kaufman, Stuart ; Fishbein, Thomas. / Nucleotide oligomerization domain 2 polymorphisms in patients with intestinal failure. In: Journal of Gastroenterology and Hepatology (Australia). 2013 ; Vol. 28, No. 2. pp. 309-313.
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AU - Guerra, Juan Francisco

AU - Zasloff, Michael

AU - Lough, Denver

AU - Abdo, Joseph

AU - Hawksworth, Jason

AU - Mastumoto, Cal

AU - Girlanda, Raffaele

AU - Island, Eddie

AU - Shetty, Kirty

AU - Kaufman, Stuart

AU - Fishbein, Thomas

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N2 - Background: Nucleotide oligomerization domain 2 (NOD2) has been associated with intestinal immunity after the discovery that its polymorphisms are linked to Crohn's disease (CD). Intestinal failure (IF) represents a wider spectrum of diseases where intestinal homeostasis has been disrupted. Aim: To evaluate the prevalence of NOD2 mutations in a population with IF as well as its association with the different conditions causing this problem. Methods: One hundred ninety-two consecutive patients with IF and 103 healthy controls were genotyped for the three most common NOD2 polymorphisms. Genotypes were compared between the groups and were related to the entities causing IF. Results: A high percentage (26%) of patients had at least one of the three most common NOD2 polymorphisms, while only a 4.8% of healthy controls had a mutant genotype. In patients with IF, specific mutations for the 702W, 908R and 1007fs alleles were 11, 5 and 12.5%, respectively, compared with 0.9% (P=0.0003), 1.9% (P=0.1) and 1.9% (P=0.001) in the control group. If we consider patients with any cause of IF other than CD, the percentage is still as high as 18.8%, with specific mutation frequencies of 7.6% (702W; P=0.01), 5.8% (908R; P=0.1) and 8.2% (1007fs; P=0.002). We could not establish an association between a NOD2 mutant genotype with any other specific clinical condition other than CD. Conclusion: Our finding supports the importance of NOD2 in the maintenance of intestinal immune homeostasis and may be important to a variety of intestinal stressors.

AB - Background: Nucleotide oligomerization domain 2 (NOD2) has been associated with intestinal immunity after the discovery that its polymorphisms are linked to Crohn's disease (CD). Intestinal failure (IF) represents a wider spectrum of diseases where intestinal homeostasis has been disrupted. Aim: To evaluate the prevalence of NOD2 mutations in a population with IF as well as its association with the different conditions causing this problem. Methods: One hundred ninety-two consecutive patients with IF and 103 healthy controls were genotyped for the three most common NOD2 polymorphisms. Genotypes were compared between the groups and were related to the entities causing IF. Results: A high percentage (26%) of patients had at least one of the three most common NOD2 polymorphisms, while only a 4.8% of healthy controls had a mutant genotype. In patients with IF, specific mutations for the 702W, 908R and 1007fs alleles were 11, 5 and 12.5%, respectively, compared with 0.9% (P=0.0003), 1.9% (P=0.1) and 1.9% (P=0.001) in the control group. If we consider patients with any cause of IF other than CD, the percentage is still as high as 18.8%, with specific mutation frequencies of 7.6% (702W; P=0.01), 5.8% (908R; P=0.1) and 8.2% (1007fs; P=0.002). We could not establish an association between a NOD2 mutant genotype with any other specific clinical condition other than CD. Conclusion: Our finding supports the importance of NOD2 in the maintenance of intestinal immune homeostasis and may be important to a variety of intestinal stressors.

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