Nucleosomes Can Form a Polar Barrier to Transcript Elongation by RNA Polymerase II

Vladimir A. Bondarenko, Louise M. Steele, Andrea Újvári, Daria A. Gaykalova, Olga I. Kulaeva, Yury S. Polikanov, Donal S. Luse, Vasily M. Studitsky

Research output: Contribution to journalArticle

Abstract

Nucleosomes uniquely positioned on high-affinity DNA sequences present a polar barrier to transcription by human and yeast RNA polymerase II (Pol II). In one transcriptional orientation, these nucleosomes provide a strong, factor- and salt-insensitive barrier at the entry into the H3/H4 tetramer that can be recapitulated without H2A/H2B dimers. The same nucleosomes transcribed in the opposite orientation form a weaker, more diffuse barrier that is largely relieved by higher salt, TFIIS, or FACT. Barrier properties are therefore dictated by both the local nucleosome structure (influenced by the strength of the histone-DNA interactions) and the location of the high-affinity DNA region within the nucleosome. Pol II transcribes DNA sequences at the entry into the tetramer much less efficiently than the same sequences located distal to the nucleosome dyad. Thus, entry into the tetramer by Pol II facilitates further transcription, perhaps due to partial unfolding of the tetramer from DNA.

Original languageEnglish (US)
Pages (from-to)469-479
Number of pages11
JournalMolecular cell
Volume24
Issue number3
DOIs
StatePublished - Nov 3 2006
Externally publishedYes

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Nucleosomes Can Form a Polar Barrier to Transcript Elongation by RNA Polymerase II'. Together they form a unique fingerprint.

  • Cite this

    Bondarenko, V. A., Steele, L. M., Újvári, A., Gaykalova, D. A., Kulaeva, O. I., Polikanov, Y. S., Luse, D. S., & Studitsky, V. M. (2006). Nucleosomes Can Form a Polar Barrier to Transcript Elongation by RNA Polymerase II. Molecular cell, 24(3), 469-479. https://doi.org/10.1016/j.molcel.2006.09.009