Nuclear translocation of the SRF co-activator MAL in cortical neurons: Role of RhoA signalling

Akiko Tabuchi, Marcel Estevez, Jennifer A. Henderson, Ruth Marx, Jun Shiota, Hiroyasu Nakano, Jay M. Baraban

Research output: Contribution to journalArticle

Abstract

Although it is well established that RhoA signaling pathways play key roles in regulating neuronal morphology, their involvement in other aspects of neuronal function has received little attention. Recent studies have elucidated a novel intracellular signaling pathway used by RhoA to elicit activation of serum response factor (SRF)-mediated transcription. In this pathway, activation of RhoA triggers nuclear translocation of the SRF co-activator, megakaryocytic acute leukemia (MAL). In assessing whether RhoA regulates transcription in neurons via this pathway, we have found that a constitutively active form of Tech (transcript-enriched in cortex and hippocampus), a RhoA guanine nucleotide exchange factor (GEF) that is expressed in forebrain neurons, stimulates SRF reporter activity in extracts of primary cortical cultures and induces nuclear translocation of MAL in cortical neurons. Both of these responses appear to be mediated by Tech's activation of RhoA as they are not mimicked by a mutant Tech construct lacking RhoA GEF activity and are blocked by C3 transferase, a selective inhibitor of RhoA. Furthermore, Tech-induced increases in SRF activity are suppressed by a dominant negative MAL construct. These findings demonstrate that RhoA signaling pathways are able to regulate transcription in neurons by triggering translocation of the SRF co-activator MAL.

Original languageEnglish (US)
Pages (from-to)169-180
Number of pages12
JournalJournal of Neurochemistry
Volume94
Issue number1
DOIs
StatePublished - 2005

Keywords

  • Megakaryocytic acute leukemia
  • Nuclear translocation
  • Primary cortical cultures
  • Rho GTPases
  • Tech
  • mDia

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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