Nuclear targeting of mutant huntingtin increases toxicity

Matthew F. Peters, Frederick C. Nucifora, Jonathan Kushi, Holly C. Seaman, Jillian K. Cooper, William J. Herring, Valina L. Dawson, Ted M. Dawson, Christopher A. Ross

Research output: Contribution to journalArticlepeer-review

161 Scopus citations

Abstract

Huntington's disease is a neurodegenerative disorder resulting from expansion of the polyglutamine region in huntingtin. Although huntingtin is normally cytoplasmic, in affected brain regions proteolytic fragments of mutant huntingtin containing the polyglutamine repeat form intranuclear inclusions. Here, we examine the contribution of nuclear localization to toxicity by transiently transfecting neuro-2a cells with an N-terminal huntingtin fragment similar in size to that believed to be present in patients. The huntingtin fragment, HD-N63, was targeted either to the cytoplasm with a nuclear export signal (NES) or to the nucleus with a nuclear localization signal (NLS). The NES decreased the number of cells with aggregates in the nucleus while an NLS had the opposite effect. By cotransfecting HD-N63 with GFP as a marker, we observed direct cell loss with constructs containing expanded polyglutamine repeats. Compared to unmodified HD-N63-75Q, adding an NES reduced cell loss by 57% while an NLS increased cell loss by 111%. These results indicate that nuclear localization of mutant huntingtin fragments plays an important role in cell toxicity.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalMolecular and Cellular Neurosciences
Volume14
Issue number2
DOIs
StatePublished - Aug 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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