TY - JOUR
T1 - Nuclear protein matrix
T2 - Association with newly synthesized DNA
AU - Berezney, Ronald
AU - Coffey, Donald S.
PY - 1975
Y1 - 1975
N2 - The residual structural framework of the cell nucleus, termed the nuclear protein matrix, is associated with newly synthesized DNA in regenerating rat liver. One minute after rats are injected with [3H]thymidine, more than 90 percent of the total tritium in nuclear DNA is associated with the matrix DNA although this DNA comprises only 25 percent of the total nuclear DNA. In contrast, the bulk DNA, 75 percent of total nuclear DNA, contains less than 8 percent of the total labeled DNA. The percentage of total labeled DNA associated with the bulk DNA increases for 30 minutes after injection and decreases correspondingly in the matrix DNA.
AB - The residual structural framework of the cell nucleus, termed the nuclear protein matrix, is associated with newly synthesized DNA in regenerating rat liver. One minute after rats are injected with [3H]thymidine, more than 90 percent of the total tritium in nuclear DNA is associated with the matrix DNA although this DNA comprises only 25 percent of the total nuclear DNA. In contrast, the bulk DNA, 75 percent of total nuclear DNA, contains less than 8 percent of the total labeled DNA. The percentage of total labeled DNA associated with the bulk DNA increases for 30 minutes after injection and decreases correspondingly in the matrix DNA.
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U2 - 10.1126/science.1145202
DO - 10.1126/science.1145202
M3 - Article
C2 - 1145202
AN - SCOPUS:0016861690
SN - 0036-8075
VL - 189
SP - 291
EP - 293
JO - Science
JF - Science
IS - 4199
ER -