Nuclear miRNA regulates the mitochondrial genome in the heart

Samarjit Das, Marcella Ferlito, Oliver A. Kent, Karen Fox-Talbot, Richard Wang, Delong Liu, Nalini Raghavachari, Yanqin Yang, Sarah Wheelan, Elizabeth Murphy, Charles Jr Steenbergen

Research output: Contribution to journalArticle

Abstract

Rationale: Mitochondria are semiautonomous cellular organelles with their own genome, which not only supply energy but also participate in cell death pathways. MicroRNAs (miRNAs) are usually 19 to 25 nt long, noncoding RNAs, involved in posttranscriptional gene regulation by binding to the 3′-untranslated regions of target mRNA, which impact on diverse cellular processes. Objective: To determine if nuclear miRNAs translocate into the mitochondria and regulate mitochondrial function with possible pathophysiological implications in cardiac myocytes. Methods and Results: We find that miR-181c is encoded in the nucleus, assembled in the cytoplasm, and finally translocated into the mitochondria of cardiac myocytes. Immunoprecipitation of Argonaute 2 from the mitochondrial fraction indicates binding of cytochrome c oxidase subunit 1 (mt-COX1) mRNA from the mitochondrial genome with miR-181c. Also, a luciferase reporter construct shows that mi-181c binds to the 3′UTR of mt-COX1. To study whether miR-181c regulates mt-COX1, we overexpressed precursor miR-181c (or a scrambled sequence) in primary cultures of neonatal rat ventricular myocytes. Overexpression of miR-181c did not change mt-COX1 mRNA but significantly decreased mt-COX1 protein, suggesting that miR-181c is primarily a translational regulator of mt-COX1. In addition to altering mt-COX1, overexpression of miR-181c results in increased mt-COX2 mRNA and protein content, with an increase in both mitochondrial respiration and reactive oxygen species generation in neonatal rat ventricular myocytes. Thus, our data show for the first time that miR-181c can enter and target the mitochondrial genome, ultimately causing electron transport chain complex IV remodeling and mitochondrial dysfunction. Conclusions: Nuclear miR-181c translocates into the mitochondria and regulates mitochondrial genome expression. This unique observation may open a new dimension to our understanding of mitochondrial dynamics and the role of miRNA in mitochondrial dysfunction.

Original languageEnglish (US)
Pages (from-to)1596-1603
Number of pages8
JournalCirculation Research
Volume110
Issue number12
DOIs
StatePublished - Jun 8 2012

Fingerprint

Mitochondrial Genome
MicroRNAs
Mitochondria
Messenger RNA
Electron Transport Complex IV
Cardiac Myocytes
Muscle Cells
Long Noncoding RNA
Mitochondrial Dynamics
3' Untranslated Regions
Luciferases
Immunoprecipitation
Organelles
Reactive Oxygen Species
Respiration
Cytoplasm
Proteins
Cell Death
Observation
Genome

Keywords

  • Cytochrome c oxidase
  • MicroRNA
  • MiR
  • Mitochondria
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Nuclear miRNA regulates the mitochondrial genome in the heart. / Das, Samarjit; Ferlito, Marcella; Kent, Oliver A.; Fox-Talbot, Karen; Wang, Richard; Liu, Delong; Raghavachari, Nalini; Yang, Yanqin; Wheelan, Sarah; Murphy, Elizabeth; Steenbergen, Charles Jr.

In: Circulation Research, Vol. 110, No. 12, 08.06.2012, p. 1596-1603.

Research output: Contribution to journalArticle

Das, S, Ferlito, M, Kent, OA, Fox-Talbot, K, Wang, R, Liu, D, Raghavachari, N, Yang, Y, Wheelan, S, Murphy, E & Steenbergen, CJ 2012, 'Nuclear miRNA regulates the mitochondrial genome in the heart', Circulation Research, vol. 110, no. 12, pp. 1596-1603. https://doi.org/10.1161/CIRCRESAHA.112.267732
Das S, Ferlito M, Kent OA, Fox-Talbot K, Wang R, Liu D et al. Nuclear miRNA regulates the mitochondrial genome in the heart. Circulation Research. 2012 Jun 8;110(12):1596-1603. https://doi.org/10.1161/CIRCRESAHA.112.267732
Das, Samarjit ; Ferlito, Marcella ; Kent, Oliver A. ; Fox-Talbot, Karen ; Wang, Richard ; Liu, Delong ; Raghavachari, Nalini ; Yang, Yanqin ; Wheelan, Sarah ; Murphy, Elizabeth ; Steenbergen, Charles Jr. / Nuclear miRNA regulates the mitochondrial genome in the heart. In: Circulation Research. 2012 ; Vol. 110, No. 12. pp. 1596-1603.
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AU - Das, Samarjit

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AU - Kent, Oliver A.

AU - Fox-Talbot, Karen

AU - Wang, Richard

AU - Liu, Delong

AU - Raghavachari, Nalini

AU - Yang, Yanqin

AU - Wheelan, Sarah

AU - Murphy, Elizabeth

AU - Steenbergen, Charles Jr

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KW - Reactive oxygen species

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