Nuclear membrane protein emerin: Roles in gene regulation, actin dynamics and human disease

Katherine Lee Wilson, James M. Holaska, Rocio Montes De Oca, Kathryn Tifft, Michael Zastrow, Miriam Segura-Totten, Malini Mansharamani, Luiza Bengtsson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Loss of emerin, a nuclear membrane protein, causes Emery-Dreifuss muscular dystrophy (EDMD), characterized by muscle weakening, contractures of major tendons and potentially lethal cardiac conduction system defects. Emerin has a LEM-domain and therefore binds barrier-to-autointegration factor (BAF), a conserved chromatin protein essential for cell division. BAF recruits emerin to chromatin and regulates higher-order chromatin structure during nuclear assembly. Emerin also binds filaments formed by A-type lamins, mutations in which also cause EDMD. Other partners for emerin include nesprin-1α and transcriptional regulators such as germ cell-less (GCL). The binding affinities of these partners range from 4 nM (nesprin-1α) to 200 nM (BAF), and are physiologically significant. Biochemical studies therefore provide a valid means to predict the properties of emerin-lamin complexes in vivo. Emerin and lamin A together form stable complexes with either BAF or GCL in vitro. BAF, however, competes with GCL for binding to emerin in vitro. These and additional partners, notably actin and nuclear myosin I, suggest disease-relevant roles for emerin in gene regulation and the mechanical integrity of the nucleus.

Original languageEnglish (US)
Title of host publicationNovartis Foundation Symposium
Pages51-58
Number of pages8
Volume264
StatePublished - 2005

Publication series

NameNovartis Foundation Symposium
Volume264
ISSN (Print)15282511

Fingerprint

Nuclear Envelope
Nuclear Proteins
Actins
Membrane Proteins
Genes
Emery-Dreifuss Muscular Dystrophy
Germ Cells
Lamin Type A
Chromatin
Myosin Type I
Lamins
emerin
Contracture
Cell Division
Tendons
Muscles
Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wilson, K. L., Holaska, J. M., De Oca, R. M., Tifft, K., Zastrow, M., Segura-Totten, M., ... Bengtsson, L. (2005). Nuclear membrane protein emerin: Roles in gene regulation, actin dynamics and human disease. In Novartis Foundation Symposium (Vol. 264, pp. 51-58). (Novartis Foundation Symposium; Vol. 264).

Nuclear membrane protein emerin : Roles in gene regulation, actin dynamics and human disease. / Wilson, Katherine Lee; Holaska, James M.; De Oca, Rocio Montes; Tifft, Kathryn; Zastrow, Michael; Segura-Totten, Miriam; Mansharamani, Malini; Bengtsson, Luiza.

Novartis Foundation Symposium. Vol. 264 2005. p. 51-58 (Novartis Foundation Symposium; Vol. 264).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wilson, KL, Holaska, JM, De Oca, RM, Tifft, K, Zastrow, M, Segura-Totten, M, Mansharamani, M & Bengtsson, L 2005, Nuclear membrane protein emerin: Roles in gene regulation, actin dynamics and human disease. in Novartis Foundation Symposium. vol. 264, Novartis Foundation Symposium, vol. 264, pp. 51-58.
Wilson KL, Holaska JM, De Oca RM, Tifft K, Zastrow M, Segura-Totten M et al. Nuclear membrane protein emerin: Roles in gene regulation, actin dynamics and human disease. In Novartis Foundation Symposium. Vol. 264. 2005. p. 51-58. (Novartis Foundation Symposium).
Wilson, Katherine Lee ; Holaska, James M. ; De Oca, Rocio Montes ; Tifft, Kathryn ; Zastrow, Michael ; Segura-Totten, Miriam ; Mansharamani, Malini ; Bengtsson, Luiza. / Nuclear membrane protein emerin : Roles in gene regulation, actin dynamics and human disease. Novartis Foundation Symposium. Vol. 264 2005. pp. 51-58 (Novartis Foundation Symposium).
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