Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration

Jerry S H Lee, Christopher M. Hale, Porntula Panorchan, Shyam B. Khatau, Jerry P. George, Yiider Tseng, Colin L. Stewart, Didier Hodzic, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Lamin A/C is a major constituent of the nuclear lamina, a thin filamentous protein layer that lies beneath the nuclear envelope. Here we show that lamin A/C deficiency in mouse embryonic fibroblasts (Lmna-/- MEFs) diminishes the ability of these cells to polarize at the edge of a wound and significantly reduces cell migration speed into the wound. Moreover, lamin A/C deficiency induces significant separation of the microtubule organizing center (MTOC) from the nuclear envelope. Investigations using ballistic intracellular nanorheology reveal that lamin A/C deficiency also dramatically affects the micromechanical properties of the cytoplasm. Both the elasticity (stretchiness) and the viscosity (propensity of a material to flow) of the cytoplasm in Lmna-/- MEFs are significantly reduced. Disassembly of either the actin filament or microtubule networks in Lmna+/+ MEFs results in decrease of cytoplasmic elasticity and viscosity down to levels found in Lmna-/- MEFs. Together these results show that both the mechanical properties of the cytoskeleton and cytoskeleton-based processes, including cell motility, coupled MTOC and nucleus dynamics, and cell polarization, depend critically on the integrity of the nuclear lamina, which suggest the existence of a functional mechanical connection between the nucleus and the cytoskeleton. These results also suggest that cell polarization during cell migration requires tight mechanical coupling between MTOC and nucleus, which is mediated by lamin A/C.

Original languageEnglish (US)
Pages (from-to)2542-2552
Number of pages11
JournalBiophysical Journal
Volume93
Issue number7
DOIs
StatePublished - Oct 2007

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Lamin Type A
Mechanics
Microtubule-Organizing Center
Cytoskeleton
Nuclear Lamina
Cell Movement
Fibroblasts
Nuclear Envelope
Elasticity
Viscosity
Cytoplasm
Wounds and Injuries
Cell Nucleus
Actin Cytoskeleton
Microtubules

ASJC Scopus subject areas

  • Biophysics

Cite this

Lee, J. S. H., Hale, C. M., Panorchan, P., Khatau, S. B., George, J. P., Tseng, Y., ... Wirtz, D. (2007). Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration. Biophysical Journal, 93(7), 2542-2552. https://doi.org/10.1529/biophysj.106.102426

Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration. / Lee, Jerry S H; Hale, Christopher M.; Panorchan, Porntula; Khatau, Shyam B.; George, Jerry P.; Tseng, Yiider; Stewart, Colin L.; Hodzic, Didier; Wirtz, Denis.

In: Biophysical Journal, Vol. 93, No. 7, 10.2007, p. 2542-2552.

Research output: Contribution to journalArticle

Lee, JSH, Hale, CM, Panorchan, P, Khatau, SB, George, JP, Tseng, Y, Stewart, CL, Hodzic, D & Wirtz, D 2007, 'Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration', Biophysical Journal, vol. 93, no. 7, pp. 2542-2552. https://doi.org/10.1529/biophysj.106.102426
Lee, Jerry S H ; Hale, Christopher M. ; Panorchan, Porntula ; Khatau, Shyam B. ; George, Jerry P. ; Tseng, Yiider ; Stewart, Colin L. ; Hodzic, Didier ; Wirtz, Denis. / Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration. In: Biophysical Journal. 2007 ; Vol. 93, No. 7. pp. 2542-2552.
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