Nuclear interaction of Smac/DIABLO with Survivin at G2/M arrest prompts docetaxel-induced apoptosis in DU145 prostate cancer cells

Ji Young Kim, Jin Yong Chung, Seung Gee Lee, Yoon Jae Kim, Ji Eun Park, Ki Soo Yoo, Young Hyun Yoo, Young Chul Park, Byeong Gee Kim, Jong Min Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Smac/DIABLO is released by mitochondria in response to apoptotic stimuli and is thought to antagonize the function of inhibitors of apoptosis proteins. Recently, it has been shown that, like XIAP, Survivin can potentially interact with Smac/DIABLO. However, the precise mechanisms and cellular location of their action have not been determined. We report for the first time that Smac/DIABLO translocates to the nucleus and is colocalized with Survivin at mitotic spindles during apoptosis resulting from G2/M arrest due to docetaxel treatment of DU145 prostate cancer cells. Our data demonstrate that the nuclear interaction of Smac/DIABLO with Survivin is an important step for suppressing the anti-apoptotic function of Survivin in Doc-induced apoptosis. This suggests that the balance between cellular Smac/DIABLO and Survivin levels could be critical for cellular destiny in taxane-treated cancer cells.

Original languageEnglish (US)
Pages (from-to)949-954
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number4
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Keywords

  • Apoptosis
  • G2/M arrest
  • Mitotic apparatus
  • Prostate cancer
  • Smac
  • Survivin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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