TY - JOUR
T1 - Nuclear factor recognition sites in the gut-specific enhancer region of an Anopheles gambiae trypsin gene
AU - Shen, Zhicheng
AU - Jacobs-Lorena, Marcelo
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health (NIAID) and from the John D. and Catherine T. MacArthur Foundation.
PY - 1998/12
Y1 - 1998/12
N2 - The major digestive enzyme of Anopheles gambiae is encoded by the trypsin 1 gene. This gene is expressed exclusively in the gut and its mRNA abundance increases after ingestion of a blood meal. Previous experiments with transgenic Drosophila have shown that the enhancer region, from nucleotide -360 bp to -150 bp upstream of the transcription initiation site, is necessary to drive the gut-specific expression of a reporter gene (Skavdis et al., 1996. EMBO J. 15, 344-350). In this study, we defined DNA sequences within this region that are capable of binding nuclear factors from either gut or non-gut tissues. By electrophoretic mobility shift assays, we determined that a gut-specific nuclear factor recognizes and binds to three sites in the enhancer region with a consensus sequence TYCAAGT. Another factor, found in many tissues, recognizes and binds to at least two additional sites with a consensus sequence ACGATA. This study defines for the first time for an insect gut-specific enhancer, specific sequences that interact with nuclear factors.
AB - The major digestive enzyme of Anopheles gambiae is encoded by the trypsin 1 gene. This gene is expressed exclusively in the gut and its mRNA abundance increases after ingestion of a blood meal. Previous experiments with transgenic Drosophila have shown that the enhancer region, from nucleotide -360 bp to -150 bp upstream of the transcription initiation site, is necessary to drive the gut-specific expression of a reporter gene (Skavdis et al., 1996. EMBO J. 15, 344-350). In this study, we defined DNA sequences within this region that are capable of binding nuclear factors from either gut or non-gut tissues. By electrophoretic mobility shift assays, we determined that a gut-specific nuclear factor recognizes and binds to three sites in the enhancer region with a consensus sequence TYCAAGT. Another factor, found in many tissues, recognizes and binds to at least two additional sites with a consensus sequence ACGATA. This study defines for the first time for an insect gut-specific enhancer, specific sequences that interact with nuclear factors.
KW - Anopheles gambiae
KW - Gut-specific promoter
KW - Nuclear factors
KW - Trypsin promoter
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U2 - 10.1016/S0965-1748(98)00089-7
DO - 10.1016/S0965-1748(98)00089-7
M3 - Article
C2 - 9887516
AN - SCOPUS:0032440040
SN - 0965-1748
VL - 28
SP - 1007
EP - 1012
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 12
ER -