Nuclear envelope fission is linked to cytokinesis in budding yeast

John Lippincott, Rong Li

Research output: Contribution to journalArticle

Abstract

We have investigated the relationship between nuclear envelope fission and cytokinesis during mitotic cell division in budding yeast. By carrying out time-lapse and optical sectioning video microscopy analysis of cells that express green fluorescent protein (GFP)-tagged nuclear envelope and actomyosin ring components, we found that nuclear division is temporally coupled to cytokinesis. Light and electron microscopy analysis also showed that nuclear envelope fission and the division of the nucleoplasm are severely delayed in cytokinesis mutants, resulting in discoupling between the nuclear division cycle and the budding cycle. These results suggest that homotypic membrane fusion may be activated by components or the mechanical action of cytokinetic structures and presents a mechanism for the equal partitioning of the nucleus and the temporal coordination of this event with chromosome segregation during mitosis. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalExperimental cell research
Volume260
Issue number2
DOIs
StatePublished - Nov 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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