Abstract
In prophase cells, lamin B1 is the major component of the nuclear lamina, a filamentous network underlying the nucleoplasmic side of the nuclear membrane, whereas lamin A/C is dissociated from the scaffold. In vivo fluorescence microscopy studies have shown that, during the G2/M transition, the first gap in the nuclear envelope (NE) appears before lamin B1 disassembly and is caused by early spindle microtubules impinging on the NE. This result suggests that the mechanical tearing of the NE by microtubules plays a central role to the progression of mitosis. To investigate whether this microtubule-induced NE deformation is sufficient for NE breakdown, we assess the mechanical resilience of a reconstituted lamin B1 network. Quantitative rheological methods demonstrate that human lamin B1 filaments form stiff networks that can resist much greater deformations than those caused by microtubules impinging on the NE. Moreover, lamin B1 networks possess an elastic stiffness, which increases under tension, and an exceptional resilience against shear deformations. These results demonstrate that both mechanical tearing of the lamina and biochemical modification of lamin B1 filaments are required for NE breakdown.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43462-43467 |
| Number of pages | 6 |
| Journal | Journal of Biological Chemistry |
| Volume | 279 |
| Issue number | 42 |
| DOIs | |
| State | Published - Oct 15 2004 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Nuclear envelope breakdown requires overcoming the mechanical integrity of the nuclear lamina. / Panorchan, Porntula; Schafer, Benjamin W.; Wirtz, Denis; Tseng, Yiider.
In: Journal of Biological Chemistry, Vol. 279, No. 42, 15.10.2004, p. 43462-43467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nuclear envelope breakdown requires overcoming the mechanical integrity of the nuclear lamina
AU - Panorchan, Porntula
AU - Schafer, Benjamin W.
AU - Wirtz, Denis
AU - Tseng, Yiider
PY - 2004/10/15
Y1 - 2004/10/15
N2 - In prophase cells, lamin B1 is the major component of the nuclear lamina, a filamentous network underlying the nucleoplasmic side of the nuclear membrane, whereas lamin A/C is dissociated from the scaffold. In vivo fluorescence microscopy studies have shown that, during the G2/M transition, the first gap in the nuclear envelope (NE) appears before lamin B1 disassembly and is caused by early spindle microtubules impinging on the NE. This result suggests that the mechanical tearing of the NE by microtubules plays a central role to the progression of mitosis. To investigate whether this microtubule-induced NE deformation is sufficient for NE breakdown, we assess the mechanical resilience of a reconstituted lamin B1 network. Quantitative rheological methods demonstrate that human lamin B1 filaments form stiff networks that can resist much greater deformations than those caused by microtubules impinging on the NE. Moreover, lamin B1 networks possess an elastic stiffness, which increases under tension, and an exceptional resilience against shear deformations. These results demonstrate that both mechanical tearing of the lamina and biochemical modification of lamin B1 filaments are required for NE breakdown.
AB - In prophase cells, lamin B1 is the major component of the nuclear lamina, a filamentous network underlying the nucleoplasmic side of the nuclear membrane, whereas lamin A/C is dissociated from the scaffold. In vivo fluorescence microscopy studies have shown that, during the G2/M transition, the first gap in the nuclear envelope (NE) appears before lamin B1 disassembly and is caused by early spindle microtubules impinging on the NE. This result suggests that the mechanical tearing of the NE by microtubules plays a central role to the progression of mitosis. To investigate whether this microtubule-induced NE deformation is sufficient for NE breakdown, we assess the mechanical resilience of a reconstituted lamin B1 network. Quantitative rheological methods demonstrate that human lamin B1 filaments form stiff networks that can resist much greater deformations than those caused by microtubules impinging on the NE. Moreover, lamin B1 networks possess an elastic stiffness, which increases under tension, and an exceptional resilience against shear deformations. These results demonstrate that both mechanical tearing of the lamina and biochemical modification of lamin B1 filaments are required for NE breakdown.
UR - http://www.scopus.com/inward/record.url?scp=6344282993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=6344282993&partnerID=8YFLogxK
U2 - 10.1074/jbc.M402474200
DO - 10.1074/jbc.M402474200
M3 - Article
C2 - 15292200
AN - SCOPUS:6344282993
VL - 279
SP - 43462
EP - 43467
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 42
ER -