NPC 15669 blocks neutrophil CD18 increase and lung injury during cardiopulmonary bypass in pigs

J. M. Bator, J. R. Connor, R. M. Burch, A. M. Gillinov, K. J. Zehr, J. M. Redmond, I. C. Wilson, D. E. Cameron, A. Herskowitz

Research output: Contribution to journalArticle

Abstract

DURING cardiopulmonary bypass (CPB), neutrophils become activated due to contact with extracorporeal surfaces and binding of complement fragments C3a and C5a, leading to extravasation and subsequent tissue damage. In this study, the effects of the leumedin NPC 15669 (N-[9H-(2,7-dimethylfluorenyl-9-methoxy) carbonyl]-L-leucine), a leukocyte recruitment inhibitor, were evaluated in a pig model of CPB. NPC 15669 caused significant inhibition of CPB associated increase in CD18 upregulation, lung tissue myeloperoxidase content, and percentage wet weight compared to controls. Lung histology revealed clear airways and minimal neutrophil infiltration in treated animals vs. significant oedema and cellular infiltration in controls. It is concluded that CPB causes a dramatic increase in neutrophil CD18, and that leumedins are effective in inhibiting neutrophil activation and subsequent tissue injury when administered during CPB.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalMediators of inflammation
Volume2
Issue number2
DOIs
StatePublished - Jan 1 1993

Keywords

  • Adhesion
  • CD18
  • Cardiopulmonary bypass
  • Leumedins
  • Neutrophils

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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    Bator, J. M., Connor, J. R., Burch, R. M., Gillinov, A. M., Zehr, K. J., Redmond, J. M., Wilson, I. C., Cameron, D. E., & Herskowitz, A. (1993). NPC 15669 blocks neutrophil CD18 increase and lung injury during cardiopulmonary bypass in pigs. Mediators of inflammation, 2(2), 135-141. https://doi.org/10.1155/S0962935193000201