Abstract
Background-Alterations in K+ channel expression and gating are thought to be the major cause of action potential remodeling in heart failure (HF). We previously reported the existence of a late Na+ current (I(NaL)) in cardiomyocytes of dogs with chronic HF, which suggested the importance of the Na+ channel in this remodeling process. The present study examined whether this I(NaL) exists in cardiomyocytes isolated from normal and failing human hearts. Methods and Results-A whole-cell patch-clamp technique was used to measure ion currents in cardiomyocytes isolated from the left ventricle of explanted hearts from 10 patients with end-stage HF and from 3 normal hearts. We found I(NaL) was activated at a membrane potential of -60 mV with maximum density (0.34±0105 pA/pF) at -30 mV in cardiomyocytes of both normal and failing hearts. The steady-state availability was sigmoidal, with an averaged midpoint potential of -94±2 mV and a slope factor of 6.9±0.1 mv. The current was reversibly blocked by the Na+ channel blockers tetrodotoxin (IC50= 1.5 μmol/L) and saxitoxin (IC50=98 nmol/L) in a dose-dependent manner. Both inactivation and reactivation of IN(NaL) had an ultraslow time course (τ≃0.6 seconds) and were independent of voltage. The amplitude oaf I(NaL) was independent of the peak transient Na+ current. Conclusions- Cardiomyocytes isolated from normal and explanted failing human hearts express I(NaL) characterized by an ultraslow voltage-independent inactivation and reactivation.
Original language | English (US) |
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Pages (from-to) | 2545-2552 |
Number of pages | 8 |
Journal | Circulation |
Volume | 98 |
Issue number | 23 |
DOIs | |
State | Published - Dec 8 1998 |
Externally published | Yes |
Keywords
- Action potentials
- Heart failure
- Myocytes
- Saxitoxin
- Tetrodotoxin
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)