Novi proces samo-organizacije površinski aktivnih supstanci za dobijanje podloga sa topografskim karakteristikama na više dužinskih skala za gajenje i diferentovanje matičnih ćelija

Translated title of the contribution: Novel surfactant self-assembly process generates multi-scale surface topographies for stem cell growth and differentiation

Eric G. Xie, Colin A. Cook, Warren L Grayson, Jason J. Benkoski

Research output: Contribution to journalArticle

Abstract

Topographical features on a substrate can greatly influence stem cell fate through contact guidance. While the response of stem cells to topography at the nano-, micro-, and meso-scale has been studied extensively, little is known about the interplay of surface features acting simultaneously across multiple length scales. A limiting factor has been the availability of high throughput methods for probing the potentially unlimited parameter space. Herein we describe a facile method for rapidly generating a hierarchy of multi-scaled topographical features on polymer substrates via the self-assembly of surfactants at the monomer/water interface. Having previously assembled polydimethylsiloxane-diacrylate (PDMS-DA) into surfaces resembling multiple tissue morphologies, the current study refines this method to produce biocompatible substrates. To manage the large parameter space, we limit the scope of this study to surface features spanning nanometer (< 1 µm) and micrometer (1-50 µm) length scales, which arise both individually and in combination. Adipose-derived stem cells were plated onto five surface types and their morphology, proliferation, and osteogenic differentiation were assessed after non-inductive and osteogenic culture. We observed statistically significant differences in cellular responses to each surface. Among our observations, the increased osteogenesis of cells on surfaces with nano-scaled features superimposed over micro-scaled features suggests that such hierarchical surface structure mediates the osteogenic properties of a surface.

Original languageBosnian
Pages (from-to)69-80
Number of pages12
JournalHemijska Industrija
Volume72
Issue number2
DOIs
StatePublished - Jan 1 2018

Fingerprint

Cell growth
Surface topography
Stem cells
Surface-Active Agents
Self assembly
Surface active agents
Substrates
Polydimethylsiloxane
Surface structure
Topography
Polymers
Monomers
Throughput
Availability
Tissue
Water

Keywords

  • Adipose-derived stem cells
  • Bone tissue engineering
  • Fossilized liquid assembly
  • Multi-scale topography
  • PDMS
  • Self-assembly

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

@article{512e3459c9224656a7fdc63a799c94c5,
title = "Novi proces samo-organizacije površinski aktivnih supstanci za dobijanje podloga sa topografskim karakteristikama na više dužinskih skala za gajenje i diferentovanje matičnih ćelija",
abstract = "Topographical features on a substrate can greatly influence stem cell fate through contact guidance. While the response of stem cells to topography at the nano-, micro-, and meso-scale has been studied extensively, little is known about the interplay of surface features acting simultaneously across multiple length scales. A limiting factor has been the availability of high throughput methods for probing the potentially unlimited parameter space. Herein we describe a facile method for rapidly generating a hierarchy of multi-scaled topographical features on polymer substrates via the self-assembly of surfactants at the monomer/water interface. Having previously assembled polydimethylsiloxane-diacrylate (PDMS-DA) into surfaces resembling multiple tissue morphologies, the current study refines this method to produce biocompatible substrates. To manage the large parameter space, we limit the scope of this study to surface features spanning nanometer (< 1 µm) and micrometer (1-50 µm) length scales, which arise both individually and in combination. Adipose-derived stem cells were plated onto five surface types and their morphology, proliferation, and osteogenic differentiation were assessed after non-inductive and osteogenic culture. We observed statistically significant differences in cellular responses to each surface. Among our observations, the increased osteogenesis of cells on surfaces with nano-scaled features superimposed over micro-scaled features suggests that such hierarchical surface structure mediates the osteogenic properties of a surface.",
keywords = "Adipose-derived stem cells, Bone tissue engineering, Fossilized liquid assembly, Multi-scale topography, PDMS, Self-assembly",
author = "Xie, {Eric G.} and Cook, {Colin A.} and Grayson, {Warren L} and Benkoski, {Jason J.}",
year = "2018",
month = "1",
day = "1",
doi = "10.2298/HEMIND170508020X",
language = "Bosnian",
volume = "72",
pages = "69--80",
journal = "Hemijska Industrija",
issn = "0367-598X",
publisher = "Association of Chemical Engineers of Serbia",
number = "2",

}

TY - JOUR

T1 - Novi proces samo-organizacije površinski aktivnih supstanci za dobijanje podloga sa topografskim karakteristikama na više dužinskih skala za gajenje i diferentovanje matičnih ćelija

AU - Xie, Eric G.

AU - Cook, Colin A.

AU - Grayson, Warren L

AU - Benkoski, Jason J.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Topographical features on a substrate can greatly influence stem cell fate through contact guidance. While the response of stem cells to topography at the nano-, micro-, and meso-scale has been studied extensively, little is known about the interplay of surface features acting simultaneously across multiple length scales. A limiting factor has been the availability of high throughput methods for probing the potentially unlimited parameter space. Herein we describe a facile method for rapidly generating a hierarchy of multi-scaled topographical features on polymer substrates via the self-assembly of surfactants at the monomer/water interface. Having previously assembled polydimethylsiloxane-diacrylate (PDMS-DA) into surfaces resembling multiple tissue morphologies, the current study refines this method to produce biocompatible substrates. To manage the large parameter space, we limit the scope of this study to surface features spanning nanometer (< 1 µm) and micrometer (1-50 µm) length scales, which arise both individually and in combination. Adipose-derived stem cells were plated onto five surface types and their morphology, proliferation, and osteogenic differentiation were assessed after non-inductive and osteogenic culture. We observed statistically significant differences in cellular responses to each surface. Among our observations, the increased osteogenesis of cells on surfaces with nano-scaled features superimposed over micro-scaled features suggests that such hierarchical surface structure mediates the osteogenic properties of a surface.

AB - Topographical features on a substrate can greatly influence stem cell fate through contact guidance. While the response of stem cells to topography at the nano-, micro-, and meso-scale has been studied extensively, little is known about the interplay of surface features acting simultaneously across multiple length scales. A limiting factor has been the availability of high throughput methods for probing the potentially unlimited parameter space. Herein we describe a facile method for rapidly generating a hierarchy of multi-scaled topographical features on polymer substrates via the self-assembly of surfactants at the monomer/water interface. Having previously assembled polydimethylsiloxane-diacrylate (PDMS-DA) into surfaces resembling multiple tissue morphologies, the current study refines this method to produce biocompatible substrates. To manage the large parameter space, we limit the scope of this study to surface features spanning nanometer (< 1 µm) and micrometer (1-50 µm) length scales, which arise both individually and in combination. Adipose-derived stem cells were plated onto five surface types and their morphology, proliferation, and osteogenic differentiation were assessed after non-inductive and osteogenic culture. We observed statistically significant differences in cellular responses to each surface. Among our observations, the increased osteogenesis of cells on surfaces with nano-scaled features superimposed over micro-scaled features suggests that such hierarchical surface structure mediates the osteogenic properties of a surface.

KW - Adipose-derived stem cells

KW - Bone tissue engineering

KW - Fossilized liquid assembly

KW - Multi-scale topography

KW - PDMS

KW - Self-assembly

UR - http://www.scopus.com/inward/record.url?scp=85047059227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047059227&partnerID=8YFLogxK

U2 - 10.2298/HEMIND170508020X

DO - 10.2298/HEMIND170508020X

M3 - Article

AN - SCOPUS:85047059227

VL - 72

SP - 69

EP - 80

JO - Hemijska Industrija

JF - Hemijska Industrija

SN - 0367-598X

IS - 2

ER -