Novel serum proteomic signatures in a non-human primate model of retinal injury

Jeffrey J. Dunmire, Rachida Bouhenni, Michael L. Hart, Bassam T. Wakim, Anthony M. Chomyk, Sarah E. Scott, Hiroshi Nakamura, Deepak P. Edward

Research output: Contribution to journalArticle

Abstract

Purpose: To identify candidate protein biomarkers in sera indicative of acute retinal injury. Methods: We used laser photocoagulation as a model of acute retinal injury in Rhesus macaques. In a paired-control study design, we collected serum from each animal (n=6) at 4 h, 1 day, and 3 days following a mock procedure and then again following retinal laser treatment that produced mild lesions. Samples were fractionated by isoelectric focusing, digested with trypsin, and analyzed by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Spectral counting was used to determine relative protein abundances and identify proteins with statistically significant differences between control and treated sera. Results: Mild retinal injury was confirmed by fundus photography and histological examination. The average number of total proteins detected by LC-MS/MS was 908±82 among samples from all three time points. Following statistical analysis and employing stringent filtering criteria, a total of 19 proteins were identified as being significantly more abundant in sera following laser-induced retinal injury, relative to control sera. Many of the proteins detected were unique to one time point. However, four proteins (phosphoglycerate kinase 1, keratin 18, Lewis alpha-3-fucosyltransferase, and ephrin receptor A2) showed differences that were significant at both 4 h and 1 day after laser treatment, followed by a decrease to baseline levels by day 3. Conclusions: A serum biomarker response to mild retinal laser injury was demonstrated in a primate model. Among the proteins detected with highest significant differences, most are upregulated within 24 h, and their appearance in the serum is transient. It is conceivable that a panel of these proteins could provide a means for detecting the acute-phase response to retinal injury. Further investigation of these candidate biomarkers and their correlation to retinal damage is warranted.

Original languageEnglish (US)
Pages (from-to)771-779
Number of pages9
JournalMolecular Vision
Volume17
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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    Dunmire, J. J., Bouhenni, R., Hart, M. L., Wakim, B. T., Chomyk, A. M., Scott, S. E., Nakamura, H., & Edward, D. P. (2011). Novel serum proteomic signatures in a non-human primate model of retinal injury. Molecular Vision, 17, 771-779.