Novel roles for selected genes in meiotic DNA processing.

Philip Jordan, Franz Klein, David R.F. Leach

Research output: Contribution to journalArticle

Abstract

High-throughput studies of the 6,200 genes of Saccharomyces cerevisiae have provided valuable data resources. However, these resources require a return to experimental analysis to test predictions. An in-silico screen, mining existing interaction, expression, localization, and phenotype datasets was developed with the aim of selecting minimally characterized genes involved in meiotic DNA processing. Based on our selection procedure, 81 deletion mutants were constructed and tested for phenotypic abnormalities. Eleven (13.6%) genes were identified to have novel roles in meiotic DNA processes including DNA replication, recombination, and chromosome segregation. In particular, this analysis showed that Def1, a protein that facilitates ubiquitination of RNA polymerase II as a response to DNA damage, is required for efficient synapsis between homologues and normal levels of crossover recombination during meiosis. These characteristics are shared by a group of proteins required for Zip1 loading (ZMM proteins). Additionally, Soh1/Med31, a subunit of the RNA pol II mediator complex, Bre5, a ubiquitin protease cofactor and an uncharacterized protein, Rmr1/Ygl250w, are required for normal levels of gene conversion events during meiosis. We show how existing datasets may be used to define gene sets enriched for specific roles and how these can be evaluated by experimental analysis.

Original languageEnglish (US)
JournalPLoS Genetics
Volume3
Issue number12
DOIs
StatePublished - Dec 2007
Externally publishedYes

Fingerprint

DNA
RNA Polymerase II
gene
Meiosis
protein
meiosis
Genetic Recombination
Genes
Proteins
genes
proteins
Mediator Complex
recombination
RNA
Chromosome Pairing
Gene Conversion
Chromosome Segregation
gene conversion
chromosome segregation
Ubiquitination

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Novel roles for selected genes in meiotic DNA processing. / Jordan, Philip; Klein, Franz; Leach, David R.F.

In: PLoS Genetics, Vol. 3, No. 12, 12.2007.

Research output: Contribution to journalArticle

Jordan, Philip ; Klein, Franz ; Leach, David R.F. / Novel roles for selected genes in meiotic DNA processing. In: PLoS Genetics. 2007 ; Vol. 3, No. 12.
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