TY - JOUR
T1 - Novel recombinant RD2- and RD11-encoded Mycobacterium tuberculosis antigens are potential candidates for diagnosis of tuberculosis infections in BCG-vaccinated individuals
AU - Chen, Jiazhen
AU - Su, Xiaodi
AU - Zhang, Ying
AU - Wang, Sen
AU - Shao, Linyun
AU - Wu, Jing
AU - Wang, Feifei
AU - Zhang, Shu
AU - Wang, Jiuling
AU - Weng, Xinhua
AU - Wang, Honghai
AU - Zhang, Wenhong
N1 - Funding Information:
We express our appreciation to the staff of the No. 5 Hospital of Suzhou, Chongqing Pulmonary Hospital and Shandong Chest Hospital for their dedication to our study. This work was supported by National 11th five-year Key Technologies Research and Development Program of China (2008ZX10003003, 2008ZX10003011), and the Major State Basic Research (973) Program of China (2005CB523102).
PY - 2009/9
Y1 - 2009/9
N2 - Proteins encoded by region of deletions (RD) of Mycobacterium tuberculosis are useful in development of vaccines and diagnostic reagents. In the present study, six M. tuberculosis genes from RD2 and RD11, rv1978, nrdf1, mpt64, cfp-21, ppe57 and ppe59, were cloned and overexpressed in Escherichia coli. All six purified recombinant proteins could distinguish tuberculosis (TB) patients and latent TB infected subjects (LTBI), or called subclinical TB infection, from BCG-vaccinated healthy controls by T-cell IFN-γ releasing ELISPOT. ELISPOT of Rv1978, NrdF1, Mpt64, CFP-21, Ppe57 and Ppe59 achieved sensitivities of 59%, 60%, 82%, 48%, 59% and 47% respectively in the detection of active TB and specificities of 94%, 90%, 76%, 93%, 100% and 93% respectively in BCG-vaccinated healthy controls. Combination of Ppe57 or NrdF1 with early secreted antigen target 6 (ESAT-6) or 10-kDa culture filtrate protein (CFP-10) in the IFN-γ releasing ESLIPOT assay could increase the sensitivities in detecting active TB, for ESAT-6 from 82.1% to 85.7% or 92.9% (P = 0.5 or 0.03, respectively) and for CFP-10 from 67.9% to 78.6% or 83.9%, respectively (both P < 0.05). The high sensitivities, specificities and promising antigenic combination of NrdF1 and Ppe57 in detection of TB in BCG-vaccinated controls suggest their potential application in TB diagnosis.
AB - Proteins encoded by region of deletions (RD) of Mycobacterium tuberculosis are useful in development of vaccines and diagnostic reagents. In the present study, six M. tuberculosis genes from RD2 and RD11, rv1978, nrdf1, mpt64, cfp-21, ppe57 and ppe59, were cloned and overexpressed in Escherichia coli. All six purified recombinant proteins could distinguish tuberculosis (TB) patients and latent TB infected subjects (LTBI), or called subclinical TB infection, from BCG-vaccinated healthy controls by T-cell IFN-γ releasing ELISPOT. ELISPOT of Rv1978, NrdF1, Mpt64, CFP-21, Ppe57 and Ppe59 achieved sensitivities of 59%, 60%, 82%, 48%, 59% and 47% respectively in the detection of active TB and specificities of 94%, 90%, 76%, 93%, 100% and 93% respectively in BCG-vaccinated healthy controls. Combination of Ppe57 or NrdF1 with early secreted antigen target 6 (ESAT-6) or 10-kDa culture filtrate protein (CFP-10) in the IFN-γ releasing ESLIPOT assay could increase the sensitivities in detecting active TB, for ESAT-6 from 82.1% to 85.7% or 92.9% (P = 0.5 or 0.03, respectively) and for CFP-10 from 67.9% to 78.6% or 83.9%, respectively (both P < 0.05). The high sensitivities, specificities and promising antigenic combination of NrdF1 and Ppe57 in detection of TB in BCG-vaccinated controls suggest their potential application in TB diagnosis.
KW - ELISPOT
KW - Immunodiagnosis
KW - Latent TB infection
KW - Mycobacterium tuberculosis
KW - RD11-encoded antigens
KW - RD2-encoded antigens
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U2 - 10.1016/j.micinf.2009.05.008
DO - 10.1016/j.micinf.2009.05.008
M3 - Article
C2 - 19467342
AN - SCOPUS:68949163060
SN - 1286-4579
VL - 11
SP - 876
EP - 885
JO - Microbes and Infection
JF - Microbes and Infection
IS - 10-11
ER -