Novel particulate spin probe for targeted determination of oxygen in cells and tissues

Ramasamy P. Pandian, Narasimham L. Parinandi, Govindasamy Ilangovan, Jay L. Zweier, Periannan Kuppusamy

Research output: Contribution to journalArticle

Abstract

The synthesis and characterization of a new lithium octa-n-butoxy- substituted naphthalocyanine radical probe (LiNc-BuO) and its use in the determination of concentration of oxygen (oximetry) by electron paramagnetic resonance (EPR) spectroscopy are reported. The probe is synthesized as a needle-shaped microcrystalline particulate. The particulate shows a single-line EPR spectrum that is highly exchange-narrowed with a line-width of 210 mG. The EPR line-width is sensitive to molecular oxygen showing a linear relationship between the line-width and concentration of oxygen (pO2) with a sensitivity of 8.5 mG/mmHg. We studied a variety of physicochemical and biological properties of LiNc-BuO particulates to evaluate the suitability of the probe for in vivo oximetry. The probe is unaffected by biological oxidoreductants, stable in tissues for several months, and can be successfully internalized in cells. We used this probe to monitor changes in concentration of oxygen in the normal muscle and RIF-1 tumor tissue of mice as a function of tumor growth. The data showed a rapid decrease in the tumor pO2 with increase of tumor volume. Human arterial smooth muscle cells, upon internalization of the LiNc-BuO probe, showed a marked oxygen gradient across the cell membrane. In summary, the newly synthesized octa-n-butoxy derivative of lithium naphthalocyanine has unique properties that are useful for determining oxygen concentration in chemical and biological systems by EPR spectroscopy and also for magnetic tagging of cells.

Original languageEnglish (US)
Pages (from-to)1138-1148
Number of pages11
JournalFree Radical Biology and Medicine
Volume35
Issue number9
DOIs
StatePublished - Nov 1 2003
Externally publishedYes

Fingerprint

Electron Spin Resonance Spectroscopy
Tissue
Oxygen
Paramagnetic resonance
Tumors
Linewidth
Oximetry
Lithium
Muscle
Spectrum Analysis
Spectroscopy
Neoplasms
Molecular oxygen
Biological systems
Cell membranes
Tumor Burden
Needles
Smooth Muscle Myocytes
Cells
Cell Membrane

Keywords

  • Electron paramagnetic resonance
  • Free radical
  • Naphthalocyanine
  • Oximetry
  • Particulate
  • RIF-1 tumor
  • Spin probe

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Pandian, R. P., Parinandi, N. L., Ilangovan, G., Zweier, J. L., & Kuppusamy, P. (2003). Novel particulate spin probe for targeted determination of oxygen in cells and tissues. Free Radical Biology and Medicine, 35(9), 1138-1148. https://doi.org/10.1016/S0891-5849(03)00496-9

Novel particulate spin probe for targeted determination of oxygen in cells and tissues. / Pandian, Ramasamy P.; Parinandi, Narasimham L.; Ilangovan, Govindasamy; Zweier, Jay L.; Kuppusamy, Periannan.

In: Free Radical Biology and Medicine, Vol. 35, No. 9, 01.11.2003, p. 1138-1148.

Research output: Contribution to journalArticle

Pandian, RP, Parinandi, NL, Ilangovan, G, Zweier, JL & Kuppusamy, P 2003, 'Novel particulate spin probe for targeted determination of oxygen in cells and tissues', Free Radical Biology and Medicine, vol. 35, no. 9, pp. 1138-1148. https://doi.org/10.1016/S0891-5849(03)00496-9
Pandian, Ramasamy P. ; Parinandi, Narasimham L. ; Ilangovan, Govindasamy ; Zweier, Jay L. ; Kuppusamy, Periannan. / Novel particulate spin probe for targeted determination of oxygen in cells and tissues. In: Free Radical Biology and Medicine. 2003 ; Vol. 35, No. 9. pp. 1138-1148.
@article{81c6899a2aac45c2868b23bb3bd711fe,
title = "Novel particulate spin probe for targeted determination of oxygen in cells and tissues",
abstract = "The synthesis and characterization of a new lithium octa-n-butoxy- substituted naphthalocyanine radical probe (LiNc-BuO) and its use in the determination of concentration of oxygen (oximetry) by electron paramagnetic resonance (EPR) spectroscopy are reported. The probe is synthesized as a needle-shaped microcrystalline particulate. The particulate shows a single-line EPR spectrum that is highly exchange-narrowed with a line-width of 210 mG. The EPR line-width is sensitive to molecular oxygen showing a linear relationship between the line-width and concentration of oxygen (pO2) with a sensitivity of 8.5 mG/mmHg. We studied a variety of physicochemical and biological properties of LiNc-BuO particulates to evaluate the suitability of the probe for in vivo oximetry. The probe is unaffected by biological oxidoreductants, stable in tissues for several months, and can be successfully internalized in cells. We used this probe to monitor changes in concentration of oxygen in the normal muscle and RIF-1 tumor tissue of mice as a function of tumor growth. The data showed a rapid decrease in the tumor pO2 with increase of tumor volume. Human arterial smooth muscle cells, upon internalization of the LiNc-BuO probe, showed a marked oxygen gradient across the cell membrane. In summary, the newly synthesized octa-n-butoxy derivative of lithium naphthalocyanine has unique properties that are useful for determining oxygen concentration in chemical and biological systems by EPR spectroscopy and also for magnetic tagging of cells.",
keywords = "Electron paramagnetic resonance, Free radical, Naphthalocyanine, Oximetry, Particulate, RIF-1 tumor, Spin probe",
author = "Pandian, {Ramasamy P.} and Parinandi, {Narasimham L.} and Govindasamy Ilangovan and Zweier, {Jay L.} and Periannan Kuppusamy",
year = "2003",
month = "11",
day = "1",
doi = "10.1016/S0891-5849(03)00496-9",
language = "English (US)",
volume = "35",
pages = "1138--1148",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Novel particulate spin probe for targeted determination of oxygen in cells and tissues

AU - Pandian, Ramasamy P.

AU - Parinandi, Narasimham L.

AU - Ilangovan, Govindasamy

AU - Zweier, Jay L.

AU - Kuppusamy, Periannan

PY - 2003/11/1

Y1 - 2003/11/1

N2 - The synthesis and characterization of a new lithium octa-n-butoxy- substituted naphthalocyanine radical probe (LiNc-BuO) and its use in the determination of concentration of oxygen (oximetry) by electron paramagnetic resonance (EPR) spectroscopy are reported. The probe is synthesized as a needle-shaped microcrystalline particulate. The particulate shows a single-line EPR spectrum that is highly exchange-narrowed with a line-width of 210 mG. The EPR line-width is sensitive to molecular oxygen showing a linear relationship between the line-width and concentration of oxygen (pO2) with a sensitivity of 8.5 mG/mmHg. We studied a variety of physicochemical and biological properties of LiNc-BuO particulates to evaluate the suitability of the probe for in vivo oximetry. The probe is unaffected by biological oxidoreductants, stable in tissues for several months, and can be successfully internalized in cells. We used this probe to monitor changes in concentration of oxygen in the normal muscle and RIF-1 tumor tissue of mice as a function of tumor growth. The data showed a rapid decrease in the tumor pO2 with increase of tumor volume. Human arterial smooth muscle cells, upon internalization of the LiNc-BuO probe, showed a marked oxygen gradient across the cell membrane. In summary, the newly synthesized octa-n-butoxy derivative of lithium naphthalocyanine has unique properties that are useful for determining oxygen concentration in chemical and biological systems by EPR spectroscopy and also for magnetic tagging of cells.

AB - The synthesis and characterization of a new lithium octa-n-butoxy- substituted naphthalocyanine radical probe (LiNc-BuO) and its use in the determination of concentration of oxygen (oximetry) by electron paramagnetic resonance (EPR) spectroscopy are reported. The probe is synthesized as a needle-shaped microcrystalline particulate. The particulate shows a single-line EPR spectrum that is highly exchange-narrowed with a line-width of 210 mG. The EPR line-width is sensitive to molecular oxygen showing a linear relationship between the line-width and concentration of oxygen (pO2) with a sensitivity of 8.5 mG/mmHg. We studied a variety of physicochemical and biological properties of LiNc-BuO particulates to evaluate the suitability of the probe for in vivo oximetry. The probe is unaffected by biological oxidoreductants, stable in tissues for several months, and can be successfully internalized in cells. We used this probe to monitor changes in concentration of oxygen in the normal muscle and RIF-1 tumor tissue of mice as a function of tumor growth. The data showed a rapid decrease in the tumor pO2 with increase of tumor volume. Human arterial smooth muscle cells, upon internalization of the LiNc-BuO probe, showed a marked oxygen gradient across the cell membrane. In summary, the newly synthesized octa-n-butoxy derivative of lithium naphthalocyanine has unique properties that are useful for determining oxygen concentration in chemical and biological systems by EPR spectroscopy and also for magnetic tagging of cells.

KW - Electron paramagnetic resonance

KW - Free radical

KW - Naphthalocyanine

KW - Oximetry

KW - Particulate

KW - RIF-1 tumor

KW - Spin probe

UR - http://www.scopus.com/inward/record.url?scp=0142231339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142231339&partnerID=8YFLogxK

U2 - 10.1016/S0891-5849(03)00496-9

DO - 10.1016/S0891-5849(03)00496-9

M3 - Article

VL - 35

SP - 1138

EP - 1148

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 9

ER -