Novel liver macromolecular MR contrast agent with a polypropylenimine diaminobutyl dendrimer core: Comparison to the vascular MR contrast agent with the polyamidoamine dendrimer core

Hisataka Kobayashi, Satomi Kawamoto, Tsuneo Saga, Noriko Sato, Akira Hiraga, Takayoshi Ishimori, Yukio Akita, Marcelo H. Mamede, Junji Konishi, Kaori Togashi, Martin W. Brechbiel

Research output: Contribution to journalArticlepeer-review

Abstract

As MRI contrast agents, more hydrophobic molecules reportedly accumulate in the liver and thus are potentially useful as liver MRI contrast agents. In this study, a generation-4 polypropylenimine diaminobutane dendrimer (DAB-Am64), which is expected to be more hydrophobic than the generation-4 polyamidoamine dendrimer (PAMAM-G4D), was used to synthesize a conjugate with 2-(p-isothiocyanatobenzyl)-6-methyl-diethylenetriaminepentaacetic acid (1B4M) [DAB-Am64- (1B4M-Gd)64] for complexing Gd(III) ions. This DAB conjugate quickly accumulated in the liver and its characteristics were studied and compared with those of a PAMAM conjugate [PAMAM-G4D(1B4M-Gd)64], which is known to be a useful vascular MRI contrast agent, in regard to its availability as a liver MRI contrast agent. DAB-Am64-(1B4M-Gd)64 accumulated significantly more in the liver and less in blood than PAMAM-G4D-(1B4M-Gd)64 (P < 0.001). Contrast-enhanced MRI with DAB-Am64-(1B4MGd)64 was able to homogeneously enhance liver parenchyma and visualize both portal and hepatic veins of 0.5 mm diameter in mice. In conclusion, DAB-Am64-(1B4M-Gd)64 is a good candidate for a liver MRI contrast agent.

Original languageEnglish (US)
Pages (from-to)795-802
Number of pages8
JournalMagnetic resonance in medicine
Volume46
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Contrast media
  • Dendrimer
  • Liver
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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