Abstract
As MRI contrast agents, more hydrophobic molecules reportedly accumulate in the liver and thus are potentially useful as liver MRI contrast agents. In this study, a generation-4 polypropylenimine diaminobutane dendrimer (DAB-Am64), which is expected to be more hydrophobic than the generation-4 polyamidoamine dendrimer (PAMAM-G4D), was used to synthesize a conjugate with 2-(p-isothiocyanatobenzyl)-6-methyl-diethylenetriaminepentaacetic acid (1B4M) [DAB-Am64- (1B4M-Gd)64] for complexing Gd(III) ions. This DAB conjugate quickly accumulated in the liver and its characteristics were studied and compared with those of a PAMAM conjugate [PAMAM-G4D(1B4M-Gd)64], which is known to be a useful vascular MRI contrast agent, in regard to its availability as a liver MRI contrast agent. DAB-Am64-(1B4M-Gd)64 accumulated significantly more in the liver and less in blood than PAMAM-G4D-(1B4M-Gd)64 (P < 0.001). Contrast-enhanced MRI with DAB-Am64-(1B4MGd)64 was able to homogeneously enhance liver parenchyma and visualize both portal and hepatic veins of 0.5 mm diameter in mice. In conclusion, DAB-Am64-(1B4M-Gd)64 is a good candidate for a liver MRI contrast agent.
Original language | English (US) |
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Pages (from-to) | 795-802 |
Number of pages | 8 |
Journal | Magnetic resonance in medicine |
Volume | 46 |
Issue number | 4 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Contrast media
- Dendrimer
- Liver
- Magnetic resonance imaging
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging