Novel in vivo murine model to study islet potency: Engraftment and function

Ankit Bharat, Nicholas Benshoff, Barbara Olack, Sabarinathan Ramachandran, Niraj M. Desai, T. Mohanakumar

Research output: Contribution to journalArticle

Abstract

Standard islet potency testing uses transplantation of islets under the kidney capsule in diabetic severe combined immunodeficient (d-SCID) mice. Even though it is possible to achieve normoglycemia in the majority of recipients by this method, the surgical procedure, by itself, is technically difficult and associated with an appreciable mortality of animals. In addition, the spatially limited renal subcapsular site restricts the mass of islet tissue that can be transplanted. Matrigel basement membrane matrix (MATRIGEL), extracted from a mouse sarcoma, is rich in angiogenic growth factors and has been shown to support the growth of mammalian cells using murine models. In this report we demonstrate that subcutaneous islet transplantation with MATRIGEL can effectively achieve normoglycemia and that this is a simple and reproducible model for in vivo islet potency testing in d-SCID mice that overcomes many drawbacks of the conventional method of kidney subcapsular islet transplantation.

Original languageEnglish (US)
Pages (from-to)1627-1630
Number of pages4
JournalTransplantation
Volume79
Issue number11
DOIs
StatePublished - Jun 15 2005
Externally publishedYes

Keywords

  • Islet transplantation
  • Kidney capsule
  • Matrigel
  • Potency testing
  • Subcutaneous
  • d-SCID mice

ASJC Scopus subject areas

  • Transplantation

Fingerprint Dive into the research topics of 'Novel in vivo murine model to study islet potency: Engraftment and function'. Together they form a unique fingerprint.

  • Cite this

    Bharat, A., Benshoff, N., Olack, B., Ramachandran, S., Desai, N. M., & Mohanakumar, T. (2005). Novel in vivo murine model to study islet potency: Engraftment and function. Transplantation, 79(11), 1627-1630. https://doi.org/10.1097/01.TP.0000159145.86990.44