TY - JOUR
T1 - Novel approach for differential diagnosis of HIV infections in the face of vaccine-generated antibodies
T2 - Utility for detection of diverse HIV-1 subtypes
AU - Khurana, Surender
AU - Needham, James
AU - Park, Susan
AU - Mathieson, Bonnie
AU - Busch, Michael P.
AU - Nemo, George
AU - Nyambi, Phillipe
AU - Zolla-Pazner, Susan
AU - Laal, Suman
AU - Mulenga, Joseph
AU - Chomba, Elwyn
AU - Hunter, Eric
AU - Allen, Susan
AU - McIntyre, James
AU - Hewlett, Indira
AU - Lee, Sherwin
AU - Tang, Shixing
AU - Cowan, Elliot
AU - Beyrer, Chris
AU - Altfeld, Marcus
AU - Yu, Xu G.
AU - Tounkara, Anatole
AU - Koita, Ousmane
AU - Kamali, Anatoli
AU - Nguyen, Nga
AU - Graham, Barney S.
AU - Todd, Deborah
AU - Mugenyi, Peter
AU - Anzala, Omu
AU - Sanders, Eduard
AU - Ketter, Nzeera
AU - Fast, Patricia
AU - Golding, Hana
PY - 2006/11
Y1 - 2006/11
N2 - Because increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine- from virus-induced antibodies. Most of the currently tested vaccines contain multiple viral components. As a result, many vaccine recipients give positive results in FDA-licensed HIV serodetection tests. We have identified conserved sequences in Env-gp41 and Gag-p6, which are recognized soon after infection but are not included in most HIV vaccine candidates. A new HIV serodetection assay, the HIV-SELECTEST, was established that distinguishes between vaccine-induced antibodies and seroconversion due to true HIV infections. It is important to make this assay globally relevant, because many clinical trials are conducted around the world where most HIV infections are due to non-B subtype HIV-1. Therefore, the current study examined the reactivity of plasma samples from >3000 infections with diverse HIV subtypes worldwide. The HIV-SELECTEST performed at >99% specificity and sensitivity. Both recent and established infections with clades A, B, C, D, E, F, G, J, and CRFs were detected. Antibodies elicited by other vaccinations or infections endemic to the clinical trial sites did not react in this assay. Therefore, HIV-SELECTEST could be an important differential diagnostic tool for HIV vaccine trials, blood banks, and population screening worldwide.
AB - Because increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine- from virus-induced antibodies. Most of the currently tested vaccines contain multiple viral components. As a result, many vaccine recipients give positive results in FDA-licensed HIV serodetection tests. We have identified conserved sequences in Env-gp41 and Gag-p6, which are recognized soon after infection but are not included in most HIV vaccine candidates. A new HIV serodetection assay, the HIV-SELECTEST, was established that distinguishes between vaccine-induced antibodies and seroconversion due to true HIV infections. It is important to make this assay globally relevant, because many clinical trials are conducted around the world where most HIV infections are due to non-B subtype HIV-1. Therefore, the current study examined the reactivity of plasma samples from >3000 infections with diverse HIV subtypes worldwide. The HIV-SELECTEST performed at >99% specificity and sensitivity. Both recent and established infections with clades A, B, C, D, E, F, G, J, and CRFs were detected. Antibodies elicited by other vaccinations or infections endemic to the clinical trial sites did not react in this assay. Therefore, HIV-SELECTEST could be an important differential diagnostic tool for HIV vaccine trials, blood banks, and population screening worldwide.
KW - AIDS
KW - Clinical trials
KW - Diagnosis
KW - Epitope mapping
KW - HIV
KW - Peptides
KW - Phage display
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=33750569606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750569606&partnerID=8YFLogxK
U2 - 10.1097/01.qai.0000242465.50947.5f
DO - 10.1097/01.qai.0000242465.50947.5f
M3 - Article
C2 - 17019363
AN - SCOPUS:33750569606
SN - 1525-4135
VL - 43
SP - 304
EP - 312
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
IS - 3
ER -