TY - JOUR
T1 - Novel anisotropic engineered cardiac tissues
T2 - Studies of electrical propagation
AU - Bursac, Nenad
AU - Loo, Yihua
AU - Leong, Kam
AU - Tung, Leslie
N1 - Funding Information:
The authors acknowledge Melissa E. Irby, Evangeline Ng, and Sean Sheehy for technical help. This work was supported by NIH Grant HL66239 to L.T. and an American Heart Association, Mid-Atlantic Affiliate fellowship to N.B.
PY - 2007/10/5
Y1 - 2007/10/5
N2 - The goal of this study was to engineer cardiac tissue constructs with uniformly anisotropic architecture, and to evaluate their electrical function using multi-site optical mapping of cell membrane potentials. Anisotropic polymer scaffolds made by leaching of aligned sucrose templates were seeded with neonatal rat cardiac cells and cultured in rotating bioreactors for 6-14 days. Cells aligned and interconnected inside the scaffolds and when stimulated by a point electrode, supported macroscopically continuous, anisotropic impulse propagation. By culture day 14, the ratio of conduction velocities along vs. across cardiac fibers reached a value of 2, similar to that in native neonatal ventricles, while action potential duration and maximum capture rate, respectively, decreased to 120 ms and increased to ∼5 Hz. The shorter culture time and larger scaffold thickness were associated with increased incidence of sustained reentrant arrhythmias. In summary, this study is the first successful attempt to engineer a cm2-size, functional anisotropic cardiac tissue patch.
AB - The goal of this study was to engineer cardiac tissue constructs with uniformly anisotropic architecture, and to evaluate their electrical function using multi-site optical mapping of cell membrane potentials. Anisotropic polymer scaffolds made by leaching of aligned sucrose templates were seeded with neonatal rat cardiac cells and cultured in rotating bioreactors for 6-14 days. Cells aligned and interconnected inside the scaffolds and when stimulated by a point electrode, supported macroscopically continuous, anisotropic impulse propagation. By culture day 14, the ratio of conduction velocities along vs. across cardiac fibers reached a value of 2, similar to that in native neonatal ventricles, while action potential duration and maximum capture rate, respectively, decreased to 120 ms and increased to ∼5 Hz. The shorter culture time and larger scaffold thickness were associated with increased incidence of sustained reentrant arrhythmias. In summary, this study is the first successful attempt to engineer a cm2-size, functional anisotropic cardiac tissue patch.
KW - Anisotropic
KW - Arrhythmia
KW - Bioreactor
KW - Cardiac tissue engineering
KW - Cardiomyoplasty
KW - Electrophysiology
KW - Polymer scaffold
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U2 - 10.1016/j.bbrc.2007.07.138
DO - 10.1016/j.bbrc.2007.07.138
M3 - Article
C2 - 17689494
AN - SCOPUS:34547916003
SN - 0006-291X
VL - 361
SP - 847
EP - 853
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -