Novel animal model of calvarial defect: Part III. reconstruction of an irradiated wound with rhBMP-2

Christopher R. Kinsella, Zoe M. MacIsaac, James J. Cray, Darren M. Smith, Stephen Rottgers, Mark P. Mooney, Gregory M. Cooper, Joseph E. Losee

Research output: Contribution to journalArticle

Abstract

Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to be an effective therapy in the acute calvarial defect wound and in calvarial defects complicated by chronic scar. The authors compared the effectiveness of rhBMP-2 with the accepted standard of autologous graft for repair of irradiated calvarial defects. Methods: Nineteen adult New Zealand White rabbits underwent subtotal calvariectomy. Four days postoperatively, animals received 15 Gy to their wound. Six weeks postoperatively, scars were débrided and defects reconstructed in one of four groups: empty (n = 3), vehicle (buffer solution/absorbable collagen sponge; n = 3), cryopreserved autograft, (n = 3), or rhBMP-2 repair (rhBMP-2/absorbable collagen sponge, n = 10). Animals underwent computed tomography imaging at 0, 2, 4, and 6 weeks, followed by euthanization and histological analysis. Percent healing was determined and a 4 × 3 mixed model analysis of variance was performed on healing versus treatment group/postoperative time. Results: According to radiopacity, rhBMP-2/sponge and autografts were statistically equivalent, with 99 and 89 percent healing at 6 weeks. Empty and vehicle treatment groups, with 35 and 34 percent healing, were inferior to the rhBMP-2/sponge and autograft groups (p <0.05). Histologically, bone in the surgical control (autograft) group was less cellular and trabecular than bone formed after rhBMP-2/sponge treatment. Conclusions: rhBMP-2 therapy was as effective in reconstructing calvarial defects in the unfavorable irradiated wound as in the acute, favorable calvarial wound. Compared with cryopreserved autologous graft, rhBMP-2-regenerated bone resulted in equal defect coverage, similar thickness, and greater cellularity. Further studies are necessary to demonstrate the long-term viability and remodeling rhBMP-2/sponge-generated bone.

Original languageEnglish (US)
JournalPlastic and Reconstructive Surgery
Volume130
Issue number5
DOIs
StatePublished - Nov 2012
Externally publishedYes

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Animal Models
Porifera
Wounds and Injuries
Autografts
Bone and Bones
Cicatrix
Collagen
recombinant human bone morphogenetic protein-2
Transplants
Analysis of Variance
Buffers
Tomography
Rabbits
Control Groups
Therapeutics

ASJC Scopus subject areas

  • Surgery

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Novel animal model of calvarial defect : Part III. reconstruction of an irradiated wound with rhBMP-2. / Kinsella, Christopher R.; MacIsaac, Zoe M.; Cray, James J.; Smith, Darren M.; Rottgers, Stephen; Mooney, Mark P.; Cooper, Gregory M.; Losee, Joseph E.

In: Plastic and Reconstructive Surgery, Vol. 130, No. 5, 11.2012.

Research output: Contribution to journalArticle

Kinsella, Christopher R. ; MacIsaac, Zoe M. ; Cray, James J. ; Smith, Darren M. ; Rottgers, Stephen ; Mooney, Mark P. ; Cooper, Gregory M. ; Losee, Joseph E. / Novel animal model of calvarial defect : Part III. reconstruction of an irradiated wound with rhBMP-2. In: Plastic and Reconstructive Surgery. 2012 ; Vol. 130, No. 5.
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T2 - Part III. reconstruction of an irradiated wound with rhBMP-2

AU - Kinsella, Christopher R.

AU - MacIsaac, Zoe M.

AU - Cray, James J.

AU - Smith, Darren M.

AU - Rottgers, Stephen

AU - Mooney, Mark P.

AU - Cooper, Gregory M.

AU - Losee, Joseph E.

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