Notch Signaling Activation in Human Embryonic Stem Cells Is Required for Embryonic, but Not Trophoblastic, Lineage Commitment

Xiaobing Yu, Jizhong Zou, Zhaohui Ye, Holly Hammond, Guibin Chen, Akinori Tokunaga, Prashant Mali, Yue Ming Li, Curt Civin, Nicholas Gaiano, Linzhao Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

The Notch signaling pathway plays important roles in cell-fate determination during embryonic development and adult life. In this study, we focus on the role of Notch signaling in governing cell-fate choices in human embryonic stem cells (hESCs). Using genetic and pharmacological approaches, we achieved both blockade and conditional activation of Notch signaling in several hESC lines. We report here that activation of Notch signaling is required for undifferentiated hESCs to form the progeny of all three embryonic germ layers, but not trophoblast cells. In addition, transient Notch signaling pathway activation enhanced generation of hematopoietic cells from committed hESCs. These new insights into the roles of Notch in hESC-fate determination may help to efficiently direct hESC differentiation into therapeutically relevant cell types.

Original languageEnglish (US)
Pages (from-to)461-471
Number of pages11
JournalCell stem cell
Volume2
Issue number5
DOIs
StatePublished - May 8 2008

Keywords

  • STEMCELL

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

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