TY - JOUR
T1 - Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics
AU - Szotek, Paul P.
AU - Chang, Henry L.
AU - Brennand, Kristen
AU - Fujino, Akihiro
AU - Pieretti-Vanmarcke, Rafael
AU - Celso, Cristina Lo
AU - Dombkowski, David
AU - Preffer, Frederic
AU - Cohen, Kenneth S.
AU - Teixeira, Jose
AU - Donahoe, Patricia K.
PY - 2008/8/26
Y1 - 2008/8/26
N2 - Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.
AB - Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.
KW - Ovarian coelomic epithelium
KW - Somatic stem cells
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U2 - 10.1073/pnas.0805012105
DO - 10.1073/pnas.0805012105
M3 - Article
C2 - 18711140
AN - SCOPUS:50449108913
SN - 0027-8424
VL - 105
SP - 12469
EP - 12473
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 34
ER -